Diversity and relationships among Turkish okra germplasm by SRAP and phenotypic marker polymorphism

By O. Gulsen, Suleyman Karagul, K. Abak. Biologia, 2007

124 citations recorded by the source corpus.

Germplasm characterization is essential and molecular markers provide valuable information for breeding programs. Sequence-related amplified polymorphism (SRAP) and phenotypic markers were studied to determine diversity and relationships among 23 okra (Abelmoschus esculentus (L) Moench) genotypes. The 39 combinations of forward and reverse SRAP primers were used to evaluate the 21 Turkish and two randomly selected USA genotypes as outgroups, and produced 97 scorable markers, of which 50% was polymorphic for all 23 genotypes. Seventeen out of the 23 genotypes (74%) were distinguished from each other with mean similarity of 0.93. As to phenotypic markers, 33 heritable traits were evaluated in field with ten replications, 28 of them (85%) were found to be polymorphic. The UPGMA (unweighted-pair group method arithmetic average) dendrogram based on the 33 phenotypic markers distinguished all genotypes, but failed to detect any geographic association of okra genotypes, being consistent with previous study. It can be concluded that SRAP markers are useful for studying diversity and relationships among okra germplasm, and have potential in marker-aided selection, linkage mapping, and evolutionary studies.

Subfields: Insect Science, Plant Science

View Paper: https://www.semanticscholar.org/paper/af2e790002d987741a39a8547ca8c67676c0b234

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Insect Science

1. Milee Agarwal
2. H. Padh
3. Neeta Shrivastava

In Plant Science

1. Milee Agarwal
2. H. Padh
3. Neeta Shrivastava
4. A. Singh
5. B. Singh

Citations in the corpus

• Advances in molecular marker techniques and their applications in plant sciences (2008, methodology): “The SRAP marker system has been adapted for a variety of purposes in different crops, including map construction, gene tagging and genetic diversity studies (Gulsen et al. 2006).”
• Okra (Abelmoschus spp.) in West and Central Africa: Potential and progress on its improvement (2010, background): “Use of sequence related amplified polymorphism (SRAP) in marker aided selection (MAS) for various traits in Turkish germplasm has been suggested (Gulsen et al., 2007).”