An R2R3 MYB transcription factor associated with regulation of the anthocyanin biosynthetic pathway in Rosaceae

By K. Lin-Wang, K. Bolitho, K. Grafton, A. Kortstee, Sakuntala Karunairetnam, T. McGhie, R. Espley, R. Hellens, A. Allan. BMC Plant Biology, 2010

650 citations recorded by the source corpus.

BackgroundThe control of plant anthocyanin accumulation is via transcriptional regulation of the genes encoding the biosynthetic enzymes. A key activator appears to be an R2R3 MYB transcription factor. In apple fruit, skin anthocyanin levels are controlled by a gene called MYBA or MYB1, while the gene determining fruit flesh and foliage anthocyanin has been termed MYB10. In order to further understand tissue-specific anthocyanin regulation we have isolated orthologous MYB genes from all the commercially important rosaceous species.ResultsWe use gene specific primers to show that the three MYB activators of apple anthocyanin (MYB10/MYB1/MYBA) are likely alleles of each other. MYB transcription factors, with high sequence identity to the apple gene were isolated from across the rosaceous family (e.g. apples, pears, plums, cherries, peaches, raspberries, rose, strawberry). Key identifying amino acid residues were found in both the DNA-binding and C-terminal domains of these MYBs. The expression of these MYB10 genes correlates with fruit and flower anthocyanin levels. Their function was tested in tobacco and strawberry. In tobacco, these MYBs were shown to induce the anthocyanin pathway when co-expressed with bHLHs, while over-expression of strawberry and apple genes in the crop of origin elevates anthocyanins.ConclusionsThis family-wide study of rosaceous R2R3 MYBs provides insight into the evolution of this plant trait. It has implications for the development of new coloured fruit and flowers, as well as aiding the understanding of temporal-spatial colour change.

Subfields: Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/a91deb3581da11cfc4abbfb897442f6b1fec90e2

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Citations in the corpus

Recent advances in the transcriptional regulation of the flavonoid biosynthetic pathway. (2011, background): “This is especially true for MYB transcription factors regulating the flavonoid pathway, where specific motifs and conserved residues have been identified in anthocyanin (Lin-Wang et al., 2010) and flavonol (Czemmel et al.”
Retrotransposons Control Fruit-Specific, Cold-Dependent Accumulation of Anthocyanins in Blood Oranges[W][OA] (2012, background): “It also has a conserved sequence motif KPXPR(S/T)F in its C-terminal domain found in other R2R3 Myb regulators of anthocyanin biosynthesis (Stracke et al., 2001; Lin-Wang et al., 2010) Phylogenetic analysis revealed that Ruby clusters with members of R2R3 Myb subgroup 6, known to regulate anthocyani”
The bHLH transcription factor MdbHLH3 promotes anthocyanin accumulation and fruit colouration in response to low temperature in apples. (2012, background): “Recently, an increasing number of MYB TFs have been characterized from woody fruit trees (Allan et al. 2008; Deluc et al. 2008; Lin-Wang et al. 2010), suggesting that the MBW model operates in perennial deciduous trees.”
Recent advances on the regulation of anthocyanin synthesis in reproductive organs. (2011, background): “Interestingly, FaMYB10 from strawberry is poorly expressed until fruits were full-size and thus there is a 40,000 fold increase upon ripening and color change [75].”
Plant phenolics: recent advances on their biosynthesis, genetics, and ecophysiology. (2013, background): “[119] characterized a large number of anthocyanin-related R2R3MYB activators from Rosaceae species, with a focus on apple, and also identified a range of apple R2R3MYBs containing the EAR domain that were able to inhibit anthocyanin gene activation in a transient assay system [120].”
Anthocyanin Biosynthesis in Apple Fruit (2018, background): “MdMYB1 is located on the distal end of chromosome 9 and is responsible for fruit skin color, whereas MdMYB10, an allele of MdMYB1 (Lin-Wang et al., 2010), confers red flesh, foliage, and stems, as well as red fruit skin (Ban et al.”
Genetic diversity, linkage disequilibrium, and association mapping analyses of peach (Prunus persica) landraces in China (2012, background): “Because the expression of MYB10 genes correlated with fruit anthocyanin levels (Wang et al. 2010), hence, MYB10 was identified as the candidate gene for the red pigment in the flesh.”
A Conserved Network of Transcriptional Activators and Repressors Regulates Anthocyanin Pigmentation in Eudicots[C][W][OPEN] (2014, background): “This finding provides a mechanism that explains the expression pattern of MYB repressors in developing petunia flowers and during fruit ripening of strawberry (Aharoni et al., 2001; Lin-Wang et al., 2010) and grape (Vitis vinifera; Huang et al., 2014).”
Evolutionary and comparative analysis of MYB and bHLH plant transcription factors. (2011, background): “Furthermore, a similar intron/exon structure between maize and rice (Oryza sativa) R2R3-MYB factors is evident (Dias et al., 2003), as well as between Arabidopsis and members of the Rosaceae (Jiang et al., 2004b; Lin-Wang et al., 2010).”
Comprehending crystalline β-carotene accumulation by comparing engineered cell models and the natural carotenoid-rich system of citrus (2011, neutral): “Quantitative real-time PCR (qRT-PCR) was performed under conditions described previously (Lin-Wang et al., 2010).”