A Conserved Network of Transcriptional Activators and Repressors Regulates Anthocyanin Pigmentation in Eudicots[C][W][OPEN]
By N. Albert, K. Davies, D. Lewis, Huaibi Zhang, M. Montefiori, C. Brendolise, M. Boase, Hanh M. Ngo, P. Jameson, K. Schwinn. The Plant Cell, 2014
649 citations recorded by the source corpus.
The gene regulatory network that regulates the timing, location, and level of anthocyanin pigmentation in petunia is characterized, establishing features of the network that are functionally conserved in eudicots. A model of the activation and repression mechanisms of the anthocyanin regulatory network is presented, which includes a role for mobile regulatory proteins for pigmentation patterning. Plants require sophisticated regulatory mechanisms to ensure the degree of anthocyanin pigmentation is appropriate to myriad developmental and environmental signals. Central to this process are the activity of MYB-bHLH-WD repeat (MBW) complexes that regulate the transcription of anthocyanin genes. In this study, the gene regulatory network that regulates anthocyanin synthesis in petunia (Petunia hybrida) has been characterized. Genetic and molecular evidence show that the R2R3-MYB, MYB27, is an anthocyanin repressor that functions as part of the MBW complex and represses transcription through its C-terminal EAR motif. MYB27 targets both the anthocyanin pathway genes and basic-helix-loop-helix (bHLH) ANTHOCYANIN1 (AN1), itself an essential component of the MBW activation complex for pigmentation. Other features of the regulatory network identified include inhibition of AN1 activity by the competitive R3-MYB repressor MYBx and the activation of AN1, MYB27, and MYBx by the MBW activation complex, providing for both reinforcement and feedback regulation. We also demonstrate the intercellular movement of the WDR protein (AN11) and R3-repressor (MYBx), which may facilitate anthocyanin pigment pattern formation. The fundamental features of this regulatory network in the Asterid model of petunia are similar to those in the Rosid model of Arabidopsis thaliana and are thus likely to be widespread in the Eudicots.
Subfields: Biochemistry, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/3259e8a2354fc58610cc773d3857ee65ce84bdef
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Citations in the corpus
- MYB Transcription Factors as Regulators of Phenylpropanoid Metabolism in Plants. (2015, background): “…2011 PHZ - R2R3 Anthocyanin 6 Activator HQ116170 Albert et al., 2011 PhMYBx AtCAPRIC E (AtCPC) R3 Anthocyanin Repressor KF985022 Albert et al., 2011 Albert et al., 2014 PhMYB27 - R2R3 Anthocyanin 4 Repressor KF985023 Albert et al., 2014 Pine (P. taeda) PtMYB1 AtMYB20 R2R3 Monolignol 28 Activator…”
- Anthocyanin Biosynthesis and Degradation Mechanisms in Solanaceous Vegetables: A Review (2018, background): “The expression of SlAN1 in young tomato plants and PhAN1 in petunia plants was increased under high light exposure (Albert et al., 2014; Kiferle et al., 2015) while Lightbourn et al. (2007) did not observe any significant change in transcription of CaAN1 in pepper leaves after applying additional…”