Screening for antimicrobial activity among bacteria isolated from the Amazon Basin

By A. S. Motta, F. Cladera-Olivera, A. Brandelli. 2004

101 citations recorded by the source corpus.

Bacteria producing antimicrobial activity were identified among 86 isolates from aquatic environments of Brazilian Amazon Basin. Antimicrobial activity against at least one indicator strain was detected for 59 isolates (68.6%). Inhibitory activity was mostly against Gram-positive bacteria, such as Listeria monocytogenes and Bacillus cereus. The antimicrobial substances produced by 19 strains that showed higher inhibitory activity were partially characterized. These antimicrobial substances showed thermal resistance for up to 100°C and partial resistance to proteolytic treatment. Some antimicrobials were partially resistant to pronase E at 2 mg ml -1 or to treatment with trichloroacetic acid. Detection of antimicrobial activity on polyacrylamide gels revealed that the molecular weight of the compounds was lower than 14 kDa. Several strains showed antibacterial activity, which in some cases appear to be related to antimicrobial peptides. The potential of these microorganisms to produce useful antimicrobial compounds is great and must be better explored.

Subfields: Food Science, Microbiology, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/a5c4c33a4beec084bd6fdeda548b3ee1ae24a1ed

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Food Science

1. E. Ringø
2. A. Gálvez
3. A. Brandelli
4. H. Abriouel

In Microbiology

1. E. Ringø
2. S. Hoseinifar

In Molecular Biology

1. E. Ringø
2. A. Brandelli
3. S. Hoseinifar
4. K. Ghosh
5. A. Ray
6. G. Banerjee

Citations in the corpus

• Genus bacillus, promising probiotics in aquaculture: Aquatic animal origin, bio-active components, bioremediation and efficacy in fish and shellfish (2019, background): “, 2013) and in aquatic environments (Motta et al., 2004; Ichimatsu et al., 2000) as well as in the gastrointestinal (GI) tract”
• Keratinolytic proteases of Bacillus species isolated from the Amazon basin showing remarkable de-hairing activity (2007, background): “This potential for screening biotechnologically relevant microorganisms has been the matter of some investigation (Bastos et al. 2000; Motta et al. 2004).”
• Diversity and applications of Bacillus bacteriocins. (2011, background): “Bacillus sp. P45 isolated from the intestinal contents of the teleost fish Jaraqui (Piaractus mesopotamicus) of the Amazon basin, at central Amazonia near Manaus, Brazil (Motta et al., 2004), produces an antimicrobial peptide (P45) that is active against Gram-positive and Gram-negative bacteria, and”