Assessing shifts in microbial community structure across a range of grasslands of differing management intensity using CLPP, PLFA and community DNA techniques

By S. Grayston, C. D. Campbell, R. Bardgett, J. Mawdsley, C. Clegg, K. Ritz, B.S Griffiths, J. Rodwell, S. Edwards, W. J. Davies and 2 more. 2004

400 citations recorded by the source corpus.

Subfields: Ecology, Molecular Biology, Soil Science

View Paper: https://www.semanticscholar.org/paper/a472be3f8f9c2fc0a6b024cf11533b556403b354

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Ecology

In Molecular Biology

In Soil Science

Citations in the corpus

Soil microbial communities resistant to changes in plant functional group composition (2011, background): “(2001) also reported decreased functional diversity of the microbial community after nitrogen addition, others have reported an increase in SIR values with fertilization (Grayston et al., 2004; Lagomarsino et al., 2007).”
Factors affecting soil microbial community structure in tomato cropping systems (2010, background): “Agricultural management factors include tillage (Buckley and Schmidt, 2001a; Cookson et al., 2008), cover cropping (Carrera et al., 2007; Schutter et al., 2001), fertilizer (Grayston et al., 2004), organic amendments (Saison et al., 2006), and crop rotation (Olsson and Alström, 2000).”
Investigating microbial community structure in soils by physiological, biochemical and molecular fingerprinting methods (2006, methodology): “The first samples were collected from Fasset Hill, Sourhope (55 280300 N, 2 140 W), an upland grazed grassland at Sourhope Research Station in the Scottish Borders, 370 m above sea level (Grayston et al., 2004).”
Long‐term change in vegetation and soil microbial communities during the phased restoration of traditional meadow grassland (2008, background): “…microbial communities with high fungal:bacterial PLFA ratios, whereas 16 intensive management is associated with bacterial dominance of the microbial 17 community and low fungal:bacterial ratios (Donisson et al. 2000; Bardgett et al. 2001, 18 Bardgett & McAlister 1999; Grayston et al. 2004).”
Soil biological quality after 36 years of ley-arable cropping, permanent grassland and permanent arable cropping (2008, background): “Grayston et al. (2004) found different microbial community structure in grasslands with different management intensities.”
Choice of Methods for Soil Microbial Community Analysis: PLFA Maximizes Power Compared to CLPP and PCR-Based Approaches (2006, positive): “In one study, PLFA was demonstrated to be more sensitive than CLPP and a PCR-based method (guanine plus cytosine ratio) to changes in MCS across a gradient of grassland management intensities (Grayston et al., 2004).”
Soil microbial community structure in European forests in relation to forest type and atmospheric nitrogen deposition (2011, methodology): “Various methods are used to determine the composition of the microbial community, for instance BIOLOG microplate techniques, plate counts, community DNA techniques, fatty acid methyl ester (FAME) or PLFA analysis (Grayston et al. 2004; Han et al. 2007; Islam et al. 2009).”
Grazing history effects on above- and below-ground litter decomposition and nutrient cycling in two co-occurring grasses (2008, background): “…grazers have been found to enhance soil nitrogen availability and litter breakdown (Shariff et al. 1994; McNaughton et al. 1997; Tracy and Frank 1998; Olofsson and Oksanen 2002), with concomitant shifts in functional composition of the soil biota (Bardgett et al. 2001; Grayston et al. 2004).”