Choice of Methods for Soil Microbial Community Analysis: PLFA Maximizes Power Compared to CLPP and PCR-Based Approaches

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Crop rotational diversity enhances belowground communities and functions in an agroecosystem. (2015, methodology): “To assess microbial community structure we used phospholipid fatty acid (PLFA) analysis, which is a sensitive measure for detecting broad changes in microbial community structure in response to changes in plant communities (Ramsey et al. 2006; Smith et al. 2014).”
Evaluation of methods to estimate production, biomass and turnover of ectomycorrhizal mycelium in forests soils : A review (2013, methodology): “On the other hand, the PLFA method is more rapid and less expensive than methods based on nucleic acids (Ramsey et al., 2006; Frostegård et al., 2011).”
The fat that matters: Soil food web analysis using fatty acids and their carbon stable isotope signature (2010, background): “chloroform-fumigationextraction or DNA-based approaches (Leckie et al., 2004; Ramsey et al., 2006).”
The Various Roles of Fatty Acids (2018, methodology): “Besides being a rapid and cheap technique, PLFA could differentiate shifts in the microbial communities that were not detected by community level physiological profiling (in 14 of 32 studies) or PCR-based molecular methods (in 5 or 25 studies) [271].”