Hierarchical Action and Inhibition of Plant Dicer-Like Proteins in Antiviral Defense

By A. Déléris, Javier Gallego-Bartolomé, J. Bao, Kristin D. Kasschau, James C. Carrington, Olivier Voinnet. Science, 2006

937 citations recorded by the source corpus.

The mechanisms underlying induction and suppression of RNA silencing in the ongoing plant-virus arms race are poorly understood. We show here that virus-derived small RNAs produced by Arabidopsis Dicer-like 4 (DCL4) program an effector complex conferring antiviral immunity. Inhibition of DCL4 by a viral-encoded suppressor revealed the subordinate antiviral activity of DCL2. Accordingly, inactivating both DCL2 and DCL4 was necessary and sufficient to restore systemic infection of a suppressor-deficient virus. The effects of DCL2 were overcome by increasing viral dosage in inoculated leaves, but this could not surmount additional, non–cell autonomous effects of DCL4 specifically preventing viral unloading from the vasculature. These findings define a molecular framework for studying antiviral silencing and defense in plants.

Subfields: Biotechnology, Immunology, Plant Science

View Paper: https://www.semanticscholar.org/paper/9e386e86cbcbfab16837060c9072246f25322d8e

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Citations in the corpus

Small RNAs and Their Roles in Plant Development (2009, background): “…such that one DCL has priority at one substrate and surrogate DCLs access the substrate in the absence of the primary DCL have been documented in many instances, such as the production of viral, endogenous, and transgene siRNAs (Deleris et al. 2006, Fusaro et al. 2006, Kasschau et al. 2007).”
Evolution and Functional Diversification of MIRNA Genes (2011, background): “…of these miRNAs is dependent on DCL1, and not DCL2, which generates 22-nucleotide siRNAs from long double-stranded RNA (Park et al., 2002; Gasciolli et al., 2005; Xie et al., 2005; Bouché et al., 2006; Deleris et al., 2006; Lu et al., 2006; Montgomery et al., 2008b; Cuperus et al., 2010a).”
Widespread dynamic DNA methylation in response to biotic stress (2012, neutral): “The mutant plant lines met1-3 (17), met1-9 (51), ddm1-8 (52), ddm1-10 (53), drm1-2 drm2-2 cmt3-11 (54), ago4-2 (38), ago6-2 (13), rdr1-1 (55), rdr2-1 (55), rdr6-15 (56), drd1-5 (57), nrpd1a-4 (58), nrpd1b-11 (59), nrpd2a-2 nrpd2b-1 (60), and dcl21 dcl3-1 dcl4-2 (61) have been previously described.”
Roles of plant small RNAs in biotic stress responses. (2009, background): “Hence, only with P38-deficient Turnip crinkle virus (TCV ) (P38 is the TCV-encoded VSR) was the role of DCL2 and DCL4 and AGO1 in restricting TCV accumulation clearly appreciated (Figure 6), as was the milder AGO7 contribution to this process (20, 61).”
Cucumber mosaic virus-encoded 2b suppressor inhibits Arabidopsis Argonaute1 cleavage activity to counter plant defense. (2006, background): “Since interference with miRNA pathways is a common feature of many viral suppressors (Mallory et al. 2002; Kasschau et al. 2003; Chapman et al. 2004; Dunoyer et al. 2004; Mlotshwa et al. 2005), we hypothesized that the molecular phenotypes of FNY-infected plants could be attributed to the 2b…”
Antiviral immunity directed by small RNAs. (2007, positive): “Hence, VSR-decient Cymbidium ringspot virus and Turnip crinkle virus accumulate in vascular bundles, yet fail to unload into neighboring cells, which, although virusfree , exhibit sequence-specic resistance to secondary infection (Deleris et al., 2006; Havelda et al., 2003).”
Biogenesis, Turnover, and Mode of Action of Plant MicroRNAs[OPEN] (2013, background): “DCL family members generate small RNAs with distinct sizes: 21 nucleotides for DCL1 and DCL4, 22 nucleotides for DCL2, and 24 nucleotides for DCL3 (Xie et al., 2004, 2005b; Akbergenov et al., 2006; Deleris et al., 2006; Cuperus et al., 2010a).”
A two-hit trigger for siRNA biogenesis in plants. (2006, positive): “We note that this model postulates that the triggers for tasiRNA biogenesis and siRNA-mediated virus resistance are mechanistically similar—a postulate supported by the fact that a single Dicerlike protein, DCL4, is primarily responsible for both processes in wild-type Arabidopsis (Gasciolli et al.,”
The RNA silencing endonuclease Argonaute 2 mediates specific antiviral immunity in Drosophila melanogaster. (2006, background): “Indeed, there seems to be a differential requirement for two Dicer-like genes, DCL2 and DCL4, for local antiviral silencing and spread of the silencing signal, respectively (Deleris et al. 2006).”