A two-hit trigger for siRNA biogenesis in plants.

By Michael J. Axtell, Calvin H. Jan, R. Rajagopalan, David P. Bartel. Cell, 2006

667 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/2e363ff3c3f9be32353b7544e8db704fd58cfd90

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In Plant Science

Citations in the corpus

Sorting of small RNAs into Arabidopsis argonaute complexes is directed by the 5' terminal nucleotide. (2008, background): “miR390-mediated cleavage of TAS3 is required to initiate the production of tasiRNAs, which regulate the vegetative phase transition in Arabidopsis (Allen et al., 2005; Axtell et al., 2006; Peragine et al., 2004; Vazquez et al., 2004; Xie et al., 2005).”
Endogenous siRNA and miRNA targets identified by sequencing of the Arabidopsis degradome. (2008, background): “In cases like TAS1a-c and TAS2, for which other factors known to promote secondary siRNA formation, such as multiple miRNA complementary sites [7, 22] or AGO7 dependency [29–32], are not evident, high cleavageproduct accumulation may promote entrance to the secondary siRNA pathway.”
A MicroRNA Superfamily Regulates Nucleotide Binding Site–Leucine-Rich Repeats and Other mRNAs[W][OA] (2012, background): “OAOpen Access articles can be viewed online without a subscription. www.plantcell.org/cgi/doi/10.1105/tpc.111.095380 (PPR) and auxin response factor (ARF) genemRNAs (Axtell et al., 2006).”
Target mimicry provides a new mechanism for regulation of microRNA activity (2007, background): “It has recently been reported that a noncleavable miR-390 target site is in vitro a more efficient competitor for miR-390 compared with a cleavable substrat”
The emergence , evolution , and diversification of the miR 390-TAS 3-ARF pathway in land plants (2016, positive): “In TAS3L, only the 3’ miR390 target site is 37 cleavable and this sets the phase of tasiRNA production, giving rise to the two in-phase tasiARFs (Allen 38 et al. 2005; Axtell et al. 2006).”
Specificity of ARGONAUTE7-miR390 interaction and dual functionality in TAS3 trans-acting siRNA formation. (2008, background): “Features of the TAS3 family, including targeting by miR390, are highly conserved in land plants (Allen et al., 2005; Axtell et al., 2006, 2007; Talmor-Neiman et al., 2006).”
Applications of next-generation sequencing technologies in functional genomics. (2008, background): “The Illumina and SOLiD platforms allow for the generation of several millions of short 35-nt reads in comparison to up to 500,000 reads generated by the 454 technology [74], potentially providing even deeper coverage of small RNAs than the 454 platform.”
Biosynthesis and Regulation of Phenylpropanoids in Plants (2017, background): “…2007; Montgomery et al., 2008a). ta-siRNAs produced from AtTAS3 is triggered by 21nt miR390-mediated cleavage through “a two-hit trigger model” (Allen et al., 2005; Adenot et al., 2006; Axtell et al., 2006; Fahlgren et al., 2006; Garcia et al., 2006; Howell et al., 2007; Montgomery et al., 2008b).”
psRNATarget: a plant small RNA target analysis server (2011, background): “This so-called multiplicity of target sites is especially relevant to the biogenesis of siRNA because existence of dual target sites of a miRNA on a speciﬁc target transcript has been reported to be an effective trigger of ta-siRNA biogenesis from a TAS (transacting-siRNA) precursor gene (21,26).”
High-Throughput Sequencing of Arabidopsis microRNAs: Evidence for Frequent Birth and Death of MIRNA Genes (2007, background): “Another non-conserved miRNA, miR173, targets tasiRNA primary transcripts (TAS1 and TAS2) [34], which in turn yield siRNAs that also target several of the miR161- and miR400targeted PPR transcripts ([29]; data not shown).”
Micro RNAs and Short‐interfering RNAs in Plants (2007, background): “An initial DCL1-dependent, miRNAguided cleavage of tasiRNA primary transcript defines the 5’ and 3' ends of the transcript which can be converted into dsRNA by RDR6 and SGS3 and sets the 21-nt phase for accurate tasiRNA formation by DCL4 in Arabidopsis (Allen et al. 2005; Gasciolli et al. 2005; Yosh”
A High Quality Draft Consensus Sequence of the Genome of a Heterozygous Grapevine Variety (2007, background): “Sequences predicted to produce ta-siRNAs [81] are conserved in several plant species, grape included.”
Evolution and Functional Diversification of MIRNA Genes (2011, background): “Both TAS3 and miR390 are deeply conserved in plants, although in P. patens, both miR390 target sites are cleaved (Arazi et al., 2005; Axtell et al., 2006).”