Targeted mutagenesis in the model plant Nicotiana benthamiana using Cas9 RNA-guided endonuclease

By V. Nekrasov, B. Staskawicz, Detlef Weigel, Jonathan D. G. Jones, Sophien Kamoun. Nature Biotechnology, 2013

1,051 citations recorded by the source corpus.

Subfields: Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/9cbee48e1ecdf54c87c674bc779e8a53fe6da718

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Citations in the corpus

The CRISPR/Cas9 system produces specific and homozygous targeted gene editing in rice in one generation. (2014, background): “…editing in human cell lines, zebra fish and mouse (Cong et al., 2013; Hwang et al., 2013; Wang et al., 2013) and recently applied to gene modification in plants (Feng et al., 2013; Jiang et al., 2013; Li et al., 2013; Mao et al., 2013; Miao et al., 2013; Nekrasov et al., 2013; Shan et al., 2013).”
Genome Editing—Principles and Applications for Functional Genomics Research and Crop Improvement (2017, methodology): “…lettuce, cotton, Lotus japonicus, dandelion, flax, petunia, citrus, watermelon and mushroom (Jiang et al., 2013; Li et al., 2013; Mao et al., 2013; Nekrasov et al., 2013; Shan et al., 2013a; Brooks et al., 2014; Feng et al., 2014; Jia and Wang, 2014; Sugano et al., 2014; Fan et al., 2015;…”
The Neisseria meningitidis CRISPR-Cas9 System Enables Specific Genome Editing in Mammalian Cells (2016, neutral): “9 This system is composed of a Cas9 protein, a trans-activating CRISPR RNA (tracrRNA), and a targeting CRISPR RNA (crRNA) that can be programmed to recognize a genomic sequence via Watson-Crick base pairing.”
CRISPR/Cas Genome Editing and Precision Plant Breeding in Agriculture. (2019, background): “In 2013, three independent groups established the CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9) system for use in rice (Oryza sativa), wheat (Triticum aestivum), Nicotiana benthamiana, and Arabidopsis thaliana (73, 115, 140).”
Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins (2014, background): “2014) and plants (Li et al. 2013b; Nekrasov et al. 2013; Shan et al. 2013), rapidly catching up with their precursors, namely, zinc finger nucleases (ZFNs) (Bibikova et al.”
A CRISPR/Cas9 Toolbox for Multiplexed Plant Genome Editing and Transcriptional Regulation1[OPEN] (2015, background): “…successful and effective CRISPR/Cas9-mediated genome editing in plants (Feng et al., 2013, 2014; Jiang et al., 2013; Li et al., 2013; Mao et al., 2013; Miao et al., 2013; Nekrasov et al., 2013; Shan et al., 2013b; Xie and Yang, 2013; Fauser et al., 2014; Schiml et al., 2014; Zhou et al., 2014).”
The CRISPR/Cas9 system for plant genome editing and beyond. (2015, background): “benthamiana, wheat and sweet orange, or in an independent study involving rice, when potential off-target sites with at least a conserved 12-nt seed sequence were investigated (Feng et al., 2014; Jia and Wang, 2014; Li et al., 2013; Nekrasov et al., 2013; Shan et al., 2013; Upadhyay et al., 2013; Zh”
A CRISPR/Cas9 toolkit for multiplex genome editing in plants (2014, methodology): “The CRISPR/Cas system has been harnessed to achieve efficient genome editing in a variety of organisms, including bacteria, yeast, plants, and animals, as well as human cell lines [12-27].”
Response of plants to water stress (2014, background): “…restriction endonucleases, such as zinc finger nucleases (ZFN) or TAL-effector nucleases (TALEN; Shukla et al., 2009; Osakabe et al., 2010b; Zhang et al., 2010; Cermak et al., 2011), and more recently, the CRISPR/CAS system (Li et al., 2013; Nekrasov et al., 2013; Shan et al., 2013).”
Efficient Marker-Free Recovery of Custom Genetic Modifications with CRISPR/Cas9 in Caenorhabditis elegans (2014, background): “…in a wide range of organisms (Wang et al. 1999; Chiu et al. 2013; Cho et al. 2013; Dicarlo et al. 2013; Friedland et al. 2013; Gratz et al. 2013; Hwang et al. 2013; Jiang et al. 2013; Katic and Großhans 2013; Li et al. 2013; Lo et al. 2013; Nekrasov et al. 2013; Kim et al. 2014; Zhao et al. 2014).”