Widespread siRNA "off-target" transcript silencing mediated by seed region sequence complementarity.

By A. Jackson, J. Burchard, Janell M. Schelter, B. Chau, M. Cleary, Lee P. Lim, Peter S. Linsley. RNA: A publication of the RNA Society, 2006

1,004 citations recorded by the source corpus.

Subfields: Cancer Research, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/9ac2e6c3a6a6922cb81b6c547a9afe4121f1af73

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Citations in the corpus

Strategies for silencing human disease using RNA interference (2007, background): “The siRNA guide strand might function as a miRNA when it contains seed-sequence matches to mRNA 3′ UTR regions, which might lead to harmful OTE”
Dysregulation of cardiogenesis, cardiac conduction, and cell cycle in mice lacking miRNA-1-2. (2007, background): “However, many conclusions regarding base-pairing have been drawn from overexpression of miRNAs, in which nonphysiologic miRNA:mRNA interactions may occur and siRNA-like off-target effects may be observed (Birmingham et al., 2006; Jackson et al., 2006).”
RNAi-Based Insecticidal Crops: Potential Effects on Nontarget Species (2013, background): “This has been studied most intensively in mammals, and in mice, the immunostimulation by RNAi led to reduced lymphocytes and platelet cells, largely correlated with cytokine response to the siRNA (Judge et al. 2005).”
siRNA vs. shRNA: similarities and differences. (2009, background): “It has subsequently been shown that both siRNA and shRNA constructs with complementarity in the “seed region” can produce the same offtarget expressionprofiles, even across cell lines, independent of delivery method [165].”
Delivery of siRNA Therapeutics: Barriers and Carriers (2010, background): “siRNA may suppress off-targets due to partial nucleotide sequence match between the siRNA and off-target mRNA ( 21 ) and/or global degradation and inhibition of translation of mRNA caused by siRNA and its transfection agent (22,23).”
Expanding the MicroRNA Targeting Code: Functional Sites with Centered Pairing (2010, background): “In order to systematically analyze these sites, we therefore compiled additional array data from HeLa experiments with similarly transfected miRNAs and siRNAs (Birmingham et al., 2006; Jackson et al., 2006a; Jackson et al., 2006b; Schwarz et al., 2006; Anderson et al., 2008).”
Position-specific chemical modification of siRNAs reduces "off-target" transcript silencing. (2006, methodology): “Off-target transcript silencing is widespread and mediated largely by limited target sequence complementarity to the seed region of the siRNA guide strand (Jackson et al. 2006).”
Predicting effective microRNA target sites in mammalian mRNAs (2015, methodology): “…3 ′ -UTR isoform quantifications previously generated for HeLa cells ( Nam et al., 2014 ) using poly(A)-position profiling by sequencing (3P-seq) ( Jan et al., 2011 ), and developed our model using the dominant mRNA from the subset of genes for which ≥ 90% of the 3P-seq tags corresponded to a…”
A structural analysis of in vitro catalytic activities of hammerhead ribozymes (2007, neutral): “Furthermore, off-target effects by RNAi have been well noted [6-10].”