MicroRNAs Modulate Hematopoietic Lineage Differentiation

By Chang‐Zheng Chen, Ling Li, H. Lodish, David P. Bartel. Science, 2004

3,429 citations recorded by the source corpus.

MicroRNAs (miRNAs) are an abundant class of ∼22-nucleotide regulatory RNAs found in plants and animals. Some miRNAs of plants, Caenorhabditis elegans, and Drosophila play important gene-regulatory roles during development by pairing to target mRNAs to specify posttranscriptional repression of these messages. We identify three miRNAs that are specifically expressed in hematopoietic cells and show that their expression is dynamically regulated during early hematopoiesis and lineage commitment. One of these miRNAs, miR-181, was preferentially expressed in the B-lymphoid cells of mouse bone marrow, and its ectopic expression in hematopoietic stem/progenitor cells led to an increased fraction of B-lineage cells in both tissue-culture differentiation assays and adult mice. Our results indicate that microRNAs are components of the molecular circuitry that controls mouse hematopoiesis and suggest that other microRNAs have similar regulatory roles during other facets of vertebrate development.

Subfields: Cancer Research, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/994851ac50de5011324d89664e4f5ab4adc8a30c

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Citations in the corpus

MicroRNAs: key players in the immune system, differentiation, tumorigenesis and cell death (2008, background): “miR-181a directs hematopoietic stem cells toward differentiation into B cells (Chen et al., 2004).”
The Role of MicroRNAs in Human Diseases (2010, background): “The cellular functions of miRNAs appear to vary in eukaryotes, including regulation of leaf and flower development in plants (38) and modulation of differentiation of hematopoietic cells in mammals (39) .”
Human embryonic stem cells express a unique set of microRNAs. (2004, background): “Several mouse miRNAs including miR181 were recently shown to modulate hematopoiesis (Chen et al., 2003).”
MicroRNA-21 is an antiapoptotic factor in human glioblastoma cells. (2005, background): “In mammals, functions for specific miRNAs have been described in the processes of hematopoietic (6) and adipocyte differentiation (7) and insulin secretion (8).”
The microRNA miR-124 antagonizes the anti-neural REST/SCP1 pathway during embryonic CNS development. (2007, background): “The anti-neural function of SCP1 in the developing spinal cord and its recent identification as a down-regulated transcript by miR-124 (Lim et al. 2005; Conaco et al. 2006) led us to test whether SCP1 is a direct target of miR-124.”
Prediction and validation of microRNAs and their targets (2005, positive): “Ectopic expression of miR-181 led to hematopoietic differentiation [9], and pancreatic-islet-specific miR-375 has been shown to regu-”
Molecular basis for the recognition of primary microRNAs by the Drosha-DGCR8 complex. (2006, background): “of Primary microRNAs by the Drosha-DGCR8 Complex”
MicroRNA-21 (miR-21) post-transcriptionally downregulates tumor suppressor Pdcd4 and stimulates invasion, intravasation and metastasis in colorectal cancer (2008, neutral): “inhibiting apoptosis (Chen et al., 2004; Croce and Calin, 2005).”