Short-lived green fluorescent proteins for quantifying ubiquitin/proteasome-dependent proteolysis in living cells

By N. Dantuma, K. Lindsten, R. Glas, M. Jellne, M. Masucci. Nature Biotechnology, 2000

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Small molecules enhance autophagy and reduce toxicity in Huntington's disease models. (2007, methodology): “We retested 72 total primary assay positives, from which we identified a structurally nonredundant set of 21 SMIRs (4-24) and 12 SMERs (25-36) (Supplementary Fig.”
A ubiquitin ligase complex assembles linear polyubiquitin chains (2006, background): “Ub-GFP is a short-lived protein because of its degradation by the ubiqutin-fusion degradation (UFD) pathway (Dantuma et al, 2000).”
Lithium induces autophagy by inhibiting inositol monophosphatase (2005, methodology): “We next tested whether myo-inositol or PEI inhibited proteasome activity using HeLa cells stably expressing the UbG76V-GFP reporter (Dantuma et al., 2000), a fluorescentspecific proteasome substrate.”
Autophagy Inhibition Compromises Degradation of Ubiquitin-Proteasome Pathway Substrates (2009, neutral): “…1999; Sarkar et al., 2008). pDest-tdTomato-p62 (tdTomatop62), pEGFP-p62, pEGFP-p62ΔUBA and pEGFP-UBA (Bjorkoy et al., 2005), pEGFP-UbG76V (UbG76V-EGFP) (Dantuma et al., 2000), Ubi-R-Q112-EGFP (Verhoef et al., 2002), pcDNA.3HA-p97 (HA-p97), pcDNA.3-FLAG-p97 (FLAG-p97) (Boyault et al., 2006), pcDNA3.”