A Cysteine-rich Adipose Tissue-specific Secretory Factor Inhibits Adipocyte Differentiation*

By Kee-Hong Kim, Kichoon Lee, Y. Moon, H. Sul. Journal of Biological Chemistry, 2001

653 citations recorded by the source corpus.

A 12.5-kDa cysteine-rich adipose tissue-specific secretory factor (ADSF/resistin) is a novel secreted protein rich in serine and cysteine residues with a unique cysteine repeat motif of CX 12CX 8CXCX 3CX 10CXCXCX 9CC. A single 0.8-kilobase mRNA coding for this protein was found in various murine white adipose tissues including inguinal and epididymal fats and also in brown adipose tissue but not in any other tissues examined. Two species of mRNAs with sizes of 1.4 and 0.8 kilobases were found in rat adipose tissue. Sequence analysis indicates that this is because of two polyadenylation signals, the proximal one with the sequence AATACA with a single base mismatch from murine AATAAA and the distal consensus sequence AATAAA. The mRNA level was markedly increased during 3T3-L1 and primary preadipocyte differentiation into adipocytes. Its expression in adipose tissue is under tight nutritional and hormonal regulation; the mRNA level was very low during fasting and increased 25-fold when fasted mice were refed a high carbohydrate diet. It was also very low in adipose tissue of streptozotocin-diabetes and increased 23-fold upon insulin administration. Upon treatment with the conditioned medium from COS cells transfected with the expression vector, conversion of 3T3-L1 cells to adipocytes was inhibited by 80%. The regulated expression pattern suggesting this factor as an adipose sensor for the nutritional state of the animals and the inhibitory effect on adipocyte differentiation implicate its function as a feedback regulator of adipogenesis.

Subfields: Epidemiology, Molecular Biology, Physiology

View Paper: https://www.semanticscholar.org/paper/93d1e31cc6e1273b6546786502aa6b9ce2afc009

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Citations in the corpus

Resistin / Fizz3 expression in relation to obesity and peroxisome proliferator-activated receptor-gamma action in humans. (2001, background): “This is in direct contrast to what was reported in rodent adipose tissue (8).”
De novo characterization of Lycoris sprengeri transcriptome using Illumina GA II (2011, positive): “In original studies, RETN has been reported to be a tissue specific secretory factor in rodents (Steppan et al., 2001a; Kim et al., 2001), highly expressed in adipose tissue and participates in many metabolic pathways and inflammatory responses.”
Recent advances in the relationship between obesity, inflammation, and insulin resistance. (2006, positive): “In addition, resistin inhibited adipocyte differentiation [81].”
Myostatin Signals through a Transforming Growth Factor β-Like Signaling Pathway To Block Adipogenesis (2003, background): “secretory factor (22), and hypoxanthine phosphoribosyltransferase (HPRT) (52).”
Mechanisms of obesity-associated insulin resistance: many choices on the menu. (2007, background): “Resistin was identified in 2001 as an adipocyte-specific secreted protein whose expression is down-regulated by anti-diabetic drugs targeting the nuclear receptor peroxisome proliferator-activated receptor (PPAR ) (Holcomb et al. 2000; Kim et al. 2001; Steppan et al. 2001).”
Adipocytokines: mediators linking adipose tissue, inflammation and immunity (2006, background): “Resistin The adipocytokine resistin was discovered by three independent group”