Non-CpG methylation is prevalent in embryonic stem cells and may be mediated by DNA methyltransferase 3a.

By B. Ramsahoye, D. Biniszkiewicz, F. Lyko, Victoria H. Clark, A. Bird, R. Jaenisch. Proceedings of the National Academy of Sciences of the United States of America, 2000

1,007 citations recorded by the source corpus.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/92ca04b47fb2058a58a48a4633293c2970627072

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In Molecular Biology

1. Yi Zhang
2. Hao Wu
3. Manel Esteller
4. A. Bird
5. Alexander Meissner
6. Eric S. Lander
7. R. Jaenisch
8. Christoph Bock

Citations in the corpus

• DNA methylation presents distinct binding sites for human transcription factors (2013, background): “DOI: 10.7554/eLife.00726 11 of 16 Research article including ESCs, induced pluirpotent stem cells, germline and neural cells (Ramsahoye et al., 2000; Ziller et al., 2011); (3) we did not include in the screen any hemimethylated DNA motifs, which occurs in vivo on newly synthesized DNAs during…”
• Acute exercise remodels promoter methylation in human skeletal muscle. (2012, background): “Evidence has emerged to support a physiological role of non-CpG methylation (Lister et al., 2009; Ramsahoye et al., 2000; Woodcock et al., 1988), notably in stems cells, where drastic remodeling of the epigenome occurs (Bird, 2007).”
• Epigenetic codes for heterochromatin formation and silencing: rounding up the usual suspects. (2002, background): “Regardless of the significance of the retrograde signaling, the well-established flow ofmice (Ramsahoye et al., 2000).”
• Hydroxylation of 5-methylcytosine by TET1 promotes active DNA demethylation in the adult brain (2011, neutral): “The mammalian genome is methylated predominantly at the C5 position of cytosine bases within CpG dinucleotides (Suzuki and Bird, 2008; Zhu, 2009), whereas non-CpG methylation has been observed at relatively low levels in pluripotent stem cells (Lister et al., 2009; Ramsahoye et al., 2000).”
• DNA methylation, methyltransferases, and cancer (2001, background): “…regions of the genome contrasts with in vitro data indicating little sequence speci®city of the known DNA methyltransferases, which require only the CpG dinucleotide or in some cases a CpA and CpT dinucleotide as their recognition sequence (Bestor and Ingram, 1983; Ramsahoye et al., 2000).”
• Interchromosomal interactions and olfactory receptor choice. (2006, background): “CpA methylation has been reported previously only in ES cells, and the enzyme capable of this modification is a de novo DNA methyltransferase, Dnmt3A, an enzyme expressed in high levels in olfactory epithelium (MacDonald et al., 2005; Ramsahoye et al., 2000).”
• A histone H3 methyltransferase controls DNA methylation in Neurospora crassa (2001, neutral): “Nevertheless, the classic maintenance model does not account for some observations, such as heterogeneous methylation in cell clones, spreading of methylation and stable propagation of methylation at non-symmetrical sites, as observed in Neurospora and other eukaryote”
• DNA methylation patterns and epigenetic memory. (2002, background): “Although there is evidence for non-CpG methylation in ES cells, most probably owing to DNMT3A, which strongly methylates CpA as well as CpG (Ramsahoye et al. 2000; Gowher and Jeltsch 2001), non-CpG methylation is barely detectable in adult cells (Ramsahoye et al. 2000).”
• The mammalian epigenome. (2007, background): “It has been observed at a low frequency in the early mouse embryo (Haines et al., 2001) and embryonic stem (ES) cells, but is sig-nificantly decreased in somatic tissues (Ramsahoye et al., 2000).”
• RNA Interference: Biology, Mechanism, and Applications (2003, background): “(177) found that the genomes of embryonic stem cells but not that of somatic tissues harbored non-CpG methylation, which accounted for 15 to 20% of total cytosine methylation.”