Autophagy Is Essential for Preimplantation Development of Mouse Embryos

By Satoshi Tsukamoto, Akiko Kuma, Mirei Murakami, C. Kishi, A. Yamamoto, N. Mizushima. Science, 2008

594 citations recorded by the source corpus.

After fertilization, maternal proteins in oocytes are degraded and new proteins encoded by the zygotic genome are synthesized. We found that autophagy, a process for the degradation of cytoplasmic constituents in the lysosome, plays a critical role during this period. Autophagy was triggered by fertilization and up-regulated in early mouse embryos. Autophagy-defective oocytes derived from oocyte-specific Atg5 (autophagy-related 5) knockout mice failed to develop beyond the four- and eight-cell stages if they were fertilized by Atg5-null sperm, but could develop if they were fertilized by wild-type sperm. Protein synthesis rates were reduced in the autophagy-null embryos. Thus, autophagic degradation within early embryos is essential for preimplantation development in mammals.

Subfields: Epidemiology, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/90655d8798e40f5b7563a18f0b1a92c864eb1e14

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Epidemiology

1. N. Mizushima
2. Beth Levine
3. D. Klionsky
4. M. Komatsu
5. P. Saftig
6. E. Eskelinen
7. C. Kishi
8. I. Slaninová

In Molecular Biology

1. Beth Levine
2. N. Mizushima
3. M. Komatsu
4. E. Eskelinen
5. P. Saftig
6. J. Guan
7. H. Chae
8. Tohru Natsume

Citations in the corpus

• Nutrient-dependent mTORC1 association with the ULK1-Atg13-FIP200 complex required for autophagy. (2009, background): “Autophagy is also important for intracellular protein quality control, preimplantation development, degradation of intracellular pathogens, antigen presentation, tumor suppression, and certain types of cell death (Levine and Deretic, 2007; Mizushima et al., 2008; Tsukamoto et al., 2008).”
• Autophagy in mammalian development and differentiation (2010, background): “The earliest autophagic event in mammalian development is observed in fertilized oocyte”
• Autophagy: Renovation of Cells and Tissues (2011, background): “In mammals, fertilization induces massive autophagy, which plays an essential role in early embryogenesis (Tsukamoto et al., 2008) (Figure 4).”
• ULK-Atg13-FIP200 complexes mediate mTOR signaling to the autophagy machinery. (2009, background): “…provides energy for starved cells or allows for the balanced regulation between biogenesis and degradation of cellular structures, thereby playing essential roles for growth, survival, differentiation, and development (Neufeld and Baehrecke, 2008; Tsukamoto et al., 2008; Vellai et al., 2008).”
• ER stress: Autophagy induction, inhibition and selection (2015, background): “For example, during pre-implantation processes, cytoplasmic components of the oocytes need to be cleared, whereas post-fertilization requires removal of paternal mitochondria; both processes are mediated by autophagy.(63,64) Furthermore, energy production in newborn mice substantially depends on aut”
• Autophagy: a lysosomal degradation pathway with a central role in health and disease. (2009, background): “A recent study shows that autophagy-defective mouse eggs fertilized with autophagy-defective sperm, failed to develop beyond the four and eight cell stages [103].”