A quantitative assay for HIV DNA integration in vivo
By S. Butler, M. Hansen, Frederic D. Bushman. Nature Medicine, 2001
725 citations recorded by the source corpus.
Subfields: Epidemiology, Molecular Biology, Virology
View Paper: https://www.semanticscholar.org/paper/904a8ef8f8eeafd18fceb5f41e19064ba5f29e54
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In Epidemiology
In Molecular Biology
- Trey Ideker
- Frederic D. Bushman
- Didier Trono
- Roded Sharan
- Rob Knight
- Anastasia Baryshnikova
- Donna K. Slonim
- Tommaso Cai
Citations in the corpus
- The cytoplasmic body component TRIM5α restricts HIV-1 infection in Old World monkeys (2004, background): “, and late HIV-1 reverse transcripts with primers MH531 and MH532 and the probe LRT-”
- Broad antiretroviral defence by human APOBEC3G through lethal editing of nascent reverse transcripts (2003, methodology): “Taqman quantitative polymerase chain reaction (PCR) ( Applied Biosystem ) of virus sequences was performed with late reverse transcripts primers and prob”
- Genome-scale RNAi screen for host factors required for HIV replication. (2008, methodology): “DNA was prepared after an additional 24 hr, and viral DNA level was measured by Q-PCR as in Butler et al. (2001) and expressed as the percentage of RT product relative to the luciferase negative control.”
- Species-specific exclusion of APOBEC3G from HIV-1 virions by Vif. (2003, methodology): “Science 280,fidelity polymerase using the U5 forward primer MH531 and reverse 1880–1884.primer 46 bp 3 to the PBS, MH532 (Butler et al., 2001) through 40 Foley, G.E., Handler, A.H., Lynch, P.M., Wolman, S.R., Stulberg,cycles (95 , 5 min; 95 , 15 s; 60 , 1 min).”
- Cellular microRNAs contribute to HIV-1 latency in resting primary CD4+ T lymphocytes (2007, methodology): “The integrated HIV-1 was detected using primer pairs specific to Alu fragments, described previousl”
- Global analysis of host-pathogen interactions that regulate early stage HIV-1 replication (2008, methodology): “In addition, viral DNA integration was quantified by taking advantage of the amplification of HIV DNA sequences adjacent to cellular Alu repeats (Butler et al., 2001) (Figure 5A).”