Genome-scale RNAi screen for host factors required for HIV replication.

By Honglin Zhou, Min Xu, Qian Huang, Adam T. Gates, Xiaohua Douglas Zhang, J. Castle, Erica M. Stec, M. Ferrer, B. Strulovici, D. Hazuda and 1 more. Cell Host and Microbe, 2008

836 citations recorded by the source corpus.

Human immunodeficiency virus (HIV)-1 depends on the host cell machinery to support its replication. To discover cellular factors associated with HIV-1 replication, we conducted a genome-scale siRNA screen, revealing more than 311 host factors, including 267 that were not previously linked to HIV. Surprisingly, there was little overlap between these genes and the HIV dependency factors described recently. However, an analysis of the genes identified in both screens revealed overlaps in several of the associated pathways or protein complexes, including the SP1/mediator complex and the NF-kappaB signaling pathway. cDNAs for a subset of the identified genes were used to rescue HIV replication following knockdown of the cellular mRNA providing strong evidence that the following six genes are previously uncharacterized host factors for HIV: AKT1, PRKAA1, CD97, NEIL3, BMP2K, and SERPINB6. This study highlights both the power and shortcomings of large scale loss-of-function screens in discovering host-pathogen interactions.

Subfields: Molecular Biology, Virology

View Paper: https://www.semanticscholar.org/paper/1fb52071704721eddb423d3878d38b98512ac492

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In Molecular Biology

Citations in the corpus

Global landscape of HIV–human protein complexes (2011, background): “Recently, four RNAi screens identified host factors that have an adverse effect on HIV-1 replication when knocked dow”
Semi-supervised multi-task learning for predicting interactions between HIV-1 and human proteins (2010, background): “Recently three other functional screened human gene lists (König et al., 2008; Yeung et al., 2009; Zhou et al., 2008) related to HIV-1 become available online.”