Clinical metagenomics
By C. Chiu, Steve Miller. Nature reviews genetics, 2019
1,291 citations recorded by the source corpus.
Clinical metagenomic next-generation sequencing (mNGS), the comprehensive analysis of microbial and host genetic material (DNA and RNA) in samples from patients, is rapidly moving from research to clinical laboratories. This emerging approach is changing how physicians diagnose and treat infectious disease, with applications spanning a wide range of areas, including antimicrobial resistance, the microbiome, human host gene expression (transcriptomics) and oncology. Here, we focus on the challenges of implementing mNGS in the clinical laboratory and address potential solutions for maximizing its impact on patient care and public health. Clinical metagenomic next-generation sequencing (mNGS) is rapidly moving from bench to bedside. This Review discusses the clinical applications of mNGS, including infectious disease diagnostics, microbiome analyses, host response analyses and oncology applications. Moreover, the authors review the challenges that need to be overcome for mNGS to be successfully implemented in the clinical laboratory and propose solutions to maximize the benefits of clinical mNGS for patients.
Subfields: Molecular Biology, Molecular Medicine
View Paper: https://www.semanticscholar.org/paper/8fdae8707a6c5a5ec0e39a7bbf9ef2fba7b6790f
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In Molecular Biology
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Citations in the corpus
- Benchmarking Metagenomics Tools for Taxonomic Classification. (2019, background): “The potential application of these technologies to improve diagnostics and in public health settings has also been widely recognized (Chiu and Miller, 2019; Miller et al., 2013), and there is extensive ongoing work to overcome the challenges associated with clinical use of these approaches…”
- Critical Assessment of Metagenome Interpretation: the second round of challenges (2021, background): “Although there is great potential of clinical metagenomics for pathogen diagnostics and characterization 57 , multiple challenges still prevent its application in routine diagnostics 59 .”