Molecular identification of the CRAC channel by altered ion selectivity in a mutant of Orai

By Andriy V. Yeromin, Shenyuan L. Zhang, Weihua Jiang, Ying Yu, O. Safrina, M. Cahalan. Nature, 2006

845 citations recorded by the source corpus.

Subfields: Biochemistry, Plant Science, Sensory Systems

View Paper: https://www.semanticscholar.org/paper/8bf4f4c19cc820924cfd5ee46ed6940140cbbfc8

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Plant Science

1. R. Lewis
2. M. Prakriya

In Sensory Systems

1. R. Lewis
2. M. Prakriya
3. S. Feske
4. Anjana Rao
5. P. Hogan
6. Jie Zheng
7. M. Trudeau
8. M. Cahalan

Citations in the corpus

• Calcium signalling in lymphocyte activation and disease (2007, background): “Mutation of Glu106 in human ORAI1 and the corresponding Glu178 (described as Glu180 in Ref”
• Molecular Basis of Calcium Signaling in Lymphocytes: STIM and ORAI (2010, background): “The strict requirement for negative charges at this position in the channel is supported by the findings that ORAI1(E106A) and Drosophila Orai with the corresponding substitution fail to conduct Ca2+ (23, 24) and that a single E106Q replacement prevents Ca2+ flux when incorporated into a tandem tetr”
• The functional network of ion channels in T lymphocytes (2009, background): “By co-immunoprecipitation (87), nanometer-scale fluorescence resonance energy transfer (FRET) imaging (92–95), and most recently by biochemical analysis of binding by the purified proteins in vitro (96), the molecular interaction between STIM1 and Orai1 is convincingly shown to be a direct binding i”
• The Epithelial Cell-derived Atopic Dermatitis Cytokine TSLP Activates Neurons to Induce Itch (2013, positive): “ORAI1/NFAT Regulates TSLP Release in Keratinocytes ORAI1 was first identified as the channel that mediates storeoperated Ca influx required for NFAT-dependent cytokine expression during immune cell activation; loss of function mutations in ORAI1 and STIM1 lead to severe combined immunodeficiencies i”
• Store-Operated Calcium Channels. (2015, positive): “Close interactions between STIM and Orai in situ after store depletion have been shown by coimmunoprecipitation of Orai1 with STIM1 (287, 292, 396, 429) and STIM2 (287) and by FRET between STIM1-YFP and CFP-Orai1 (256, 260, 429).”
• Dual functions for the endoplasmic reticulum calcium sensors STIM1 and STIM2 in T cell activation and tolerance (2008, background): “Mutational and electrophysiological analyses have identified drosophila Orai and human ORAI1 as pore subunits of the CRAC channe”
• Handbook of ion channels (2015, methodology): “Direct binding of the two proteins has been demonstrated by several methods using co-immunoprecipitation [45,46,97,98], FRET microscopy [37,38], and pull-downs [40].”
• STIM1 Clusters and Activates CRAC Channels via Direct Binding of a Cytosolic Domain to Orai1 (2009, neutral): “Coimmunoprecipitation studies have been somewhat equivocal, with STIM1 and Orai1 reported to coimmunoprecipitate after store depletion ( Yeromin et al., 2006 ), before store depletion (Vig et al., 2006), or not at all (Gwack et al., 2007).”