Direct observation of the nanoscale dynamics of membrane lipids in a living cell

By Christian Eggeling, C. Ringemann, R. Medda, G. Schwarzmann, K. Sandhoff, S. Polyakova, V. Belov, B. Hein, C. Middendorff, A. Schönle and 1 more. Nature, 2009

1,499 citations recorded by the source corpus.

Subfields: Atomic and Molecular Physics, and Optics, Biophysics, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/8ac7832b7b2914d481e5c93eacbc77c6e62ef666

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In Atomic and Molecular Physics, and Optics

In Biophysics

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Citations in the corpus

Emerging roles of Wnts in the adult nervous system (2010, background): “Moreover, advanced imaging techniques, such as stimulated emission depletion (STED) far-field fluorescence nanoscop”
Anomalous transport in the crowded world of biological cells (2013, methodology): “Application of STED-FCS to the plasma membrane of living mammalian cells gives access to nanosized detection areas down to w = 15 nm in radius [190].”
Signalling ballet in space and time (2010, neutral): “, which transiently exist on ∼20 ms timescales and”
Revitalizing membrane rafts: new tools and insights (2010, background): “STED microscopy showed that sphingolipids and GPI-anchored proteins are transiently trapped in cholesterol-dependent molecular complexes in live cell”
A guide to super-resolution fluorescence microscopy (2010, methodology): “…al., 2007; Bock et al., 2007; Shroff et al., 2007; Bossi et al., 2008; Gunkel et al., 2009; Subach et al., 2009; van de Linde et al., 2009) and has been successfully used to map vertebrate kinetochore components Nägerl et al., 2008; Westphal et al., 2008; Eggeling et al., 2009; Opazo et al., 2010).”
Far-Field Optical Nanoscopy (2007, background): “The up to ∼ 70 times smaller detection areas created by STED (as compared to confocal microscopy) revealed marked differences between the diffusion of sphingo- and phospholipids [74].”
Probing protein heterogeneity in the plasma membrane using PALM and pair correlation analysis (2011, methodology): “We expressed PAGFP-tagged proteins transiently in COS-7 cells and imaged the plasma membrane using PALM.”