A System for Stable Expression of Short Interfering RNAs in Mammalian Cells

By T. Brummelkamp, René Bernards, Reuven Agami. Science, 2002

4,626 citations recorded by the source corpus.

Subfields: Cancer Research, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/896cfce06dc9f0e3dcca6d3f3099f777bdafe6eb

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Citations in the corpus

Tricellulin constitutes a novel barrier at tricellular contacts of epithelial cells (2005, methodology): “To suppress tricellulin expression levels in Eph4 cells, DNA oligonucleotides of the target sequences encoding two distinct portions of its COOHterminal tail were cloned into H1 promoter RNAi vector (Brummelkamp et al., 2002).”
Ki67 is a promising molecular target in the diagnosis of cancer (review). (2015, background): “To resolve these problems, certain studies have investigated the transfection of plasmid vectors to stably synthesize so-called “short hairpin RNAs” (shRNAs) in host cells, which makes it possible alter native cell processes (123-125).”
LPS-TLR4 Signaling to IRF-3/7 and NF-κB Involves the Toll Adapters TRAM and TRIF (2003, methodology): “This methodology has been used extensively to assess siRNA efficiency and provides a quantitative assessment of silencing efficiency (57).”
FoxM1 is required for execution of the mitotic programme and chromosome stability (2005, methodology): “Synthesis of 64-mer synthetic oligonucleotides for cloning into pSuper construct was performed as describe”
Involvement of microRNA in AU-rich element-mediated mRNA instability. (2005, methodology): “The genes encoding pre-miR16 and pre-miR15 were amplified from human genomic DNA and subcloned into pSuper vector (Brummelkamp et al., 2002).”
A silencing pathway to induce H3-K9 and H4-K20 trimethylation at constitutive heterochromatin. (2004, background): “Only ∼15 of these Su(var) genes have been identified to date, the most revealing of which include Su(var)2-5 (HP1; Eissenberg et al. 1990), Su(var)3-7 (Cleard et al. 1997), and Su(var)3-9 (Tschiersch et al. 1994).”
A lentiviral RNAi library for human and mouse genes applied to an arrayed viral high-content screen. (2006, background): “An alternative approach is to transduce mammalian cells with viruses carrying expression cassettes that encode short hairpin RNAs (shRNAs) to generate genespecific siRNAs within cells; this approach can achieve stable and highly effective gene suppression in a variety of mammalian cell types (Abbas-”
Strategies for silencing human disease using RNA interference (2007, background): “By contrast, expressed shRNAs mediate long-term, stable knockdown of their target transcripts for as long as transcription of the shRNAs takes plac”