An auxin-based degron system for the rapid depletion of proteins in nonplant cells

By K. Nishimura, T. Fukagawa, H. Takisawa, T. Kakimoto, Masato T. Kanemaki. Nature Methods, 2009

1,569 citations recorded by the source corpus.

Subfields: Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/875af8df74c6b61ef0e043a074ed2b9f8c8ec48c

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Citations in the corpus

A pathway for mitotic chromosome formation (2018, background): “In the presence of the plant F-box protein osTIR1, addition of auxin induces rapid proteasome-dependent degradation of the SMC2-mAID protein, thus disrupting both condensin I and II complexes (18, 26, 27).”
Systematic allelic analysis defines the interplay of key pathways in X chromosome inactivation (2018, methodology): “We therefore developed a Pcgf3 allele tagged with an Auxin-inducible degron 37 , on a Pcgf5 null background (Fig.”
In B cells, phosphatidylinositol 5-phosphate 4-kinase–α synthesizes PI(4,5)P2 to impact mTORC2 and Akt signaling (2016, methodology): “Here, we have more fully exploited the genetic power of DT40s to gain unprecedented insight into the physiological functions of PI5P4Kα.”
The non-catalytic role of DNA polymerase epsilon in replication initiation in human cells (2022, positive): “In agreement with these data we found that cat (1-1261) and ΔcatΔZF (1262-2157) constructs were unable to pull down POLE2.”
Targeted Protein Depletion Using the Auxin‐Inducible Degron 2 (AID2) System (2021, methodology): “…with a 7 kDa degron, mini-AID (mAID), derived from IAA17 of Arabidopsis thaliana (an AUX/IAA protein) is recognized by the SCF–OsTIR1 E3 ligase and targeted for rapid degradation (Kubota, Nishimura, Kanemaki, & Donaldson, 2013; Natsume, Kiyomitsu, Saga, & Kanemaki, 2016; Nishimura et al., 2009).”
Topologically associating domains and chromatin loops depend on cohesin and are regulated by CTCF, WAPL, and PDS5 proteins (2017, neutral): “For this purpose, we have either inactivated cohesin or CTCF by conditional proteolysis (Nishimura et al, 2009), or stabilized cohesin on DNA by depleting WAPL and its binding partners PDS5A and PDS5B (Kueng et al, 2006).”
Targeted degradation of CTCF decouples local insulation of chromosome domains from genomic compartmentalization (2017, positive): “Here we used a conditional degradation strategy in mouse embryonic stem cells (mESCs), the Auxin-Inducible Degron (AID) system (Nishimura et al., 2009), to acutely and reversibly deplete CTCF below detectable levels.”