Antiparallel Leucine Zipper-Directed Protein Reassembly: Application to the Green Fluorescent Protein
By I. Ghosh, A. D. Hamilton, L. Regan. 2000
511 citations recorded by the source corpus.
Subfields: Biotechnology, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/84e65b5eb57f93deadee8983bd67315c1a980561
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In Biotechnology
In Molecular Biology
- T. Kerppola
- Robert E. Campbell
- A. Ting
- Roger Tsien
- Jin Zhang
- T. C. Terwilliger
- Michael B. Elowitz
- Cori Bargmann
Citations in the corpus
- Protein tagging and detection with engineered self-assembling fragments of green fluorescent protein (2005, background): “One way to accomplish this might be with a split GF”
- Creating new fluorescent probes for cell biology (2002, background): “Protein–protein interactions can also be imaged by protein complementation assays, in which the potential partner proteins are fused not to FRET donors and acceptors but to complementary fragments of GF”
- Bimolecular fluorescence complementation (BiFC) analysis as a probe of protein interactions in living cells. (2008, background): “Many interactions have been visualized in Escherichia coli (6,33,69,75) Agrobacterium tumefaciens (15,113) and Bacillus subtilis (77,105).”
- GFP Reconstitution Across Synaptic Partners (GRASP) defines cell contacts and synapses in living nervous systems. (2008, methodology): “This approach is conceptually similar to split-GFP methods for determining intracellular protein-protein interactions in living cells (Ghosh et al., 2000; Zhang et al., 2004), but modifications were necessary for extracellular GFP assembly.”
- Simultaneous visualization of multiple protein interactions in living cells using multicolor fluorescence complementation analysis (2003, background): “Such bimolecular complementation provides a convenient approach for detection of protein interactions in cells if the protein fragments can associate only when they are brought together by interaction partners fused to the fragment”
- Programmable protein circuits in living cells (2018, background): “Indeed, incorporating a pair of hetero-dimerizing leucine zippers ( 39 ) in the protease and its target improved regulation (Fig.”