GFP Reconstitution Across Synaptic Partners (GRASP) defines cell contacts and synapses in living nervous systems.

By Evan H. Feinberg, Miri K VanHoven, Andrés Bendesky, George J. Wang, R. Fetter, K. Shen, Cori Bargmann. Neuron, 2008

693 citations recorded by the source corpus.

Subfields: Aging, Cellular and Molecular Neuroscience, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/70b38704da4726867e44f0bff872b5d6a06ce636

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Citations in the corpus

Reward signal in a recurrent circuit drives appetitive long-term memory formation (2015, positive): “The following antibodies were used: rabbit-anti-GFP (1:1000; A11122; Invitrogen), mouse- anti-GFP (1:200; UC Davis/NIH Neuromab Facility; clone N86/38; for GRASP), mouse anti-nc82 (1:33.3; Developmental Studies Hybridoma Bank, Univ. Iowa, Iowa City, IA, United States) (Hofbauer et al., 2009), rat-an”
Essential role of the mushroom body in context-dependent CO₂ avoidance in Drosophila. (2013, background): “GFP reconstitution across synaptic partners (GRASP [28]) experiments suggested that the biVPN connects to all types ofKCs, including a0/b0 KCs (Figures 3G, 3H, andS3).”
A pair of inhibitory neurons are required to sustain labile memory in the Drosophila mushroom body (2011, positive): “We also used GRASP to visualize contact between APL and DPM neurons using NP5288-GAL4 for APL and L0111-LexA for DPM (Figure 4E; Figure S2; Movie S2).”
Converging circuits mediate temperature and shock aversive olfactory conditioning in Drosophila. (2014, positive): “In order to further explore these contacts, we used GFP reconstitution across synaptic partners (GRASP) [30].”
A Transparent Window into Biology: A Primer on Caenorhabditis elegans (2015, background): “Furthermore, mapping of cell-cell and synaptic contacts can be accomplished by expressing complementary fragments of GFP in different cells (GRASP; Feinberg et al. 2008).”
Experience-dependent structural synaptic plasticity in the mammalian brain (2009, background): “, perhaps using different coloured fluorescent proteins or fluorescent indicators of synaptic contac”
γ neurons mediate dopaminergic input during aversive olfactory memory formation in Drosophila (2012, positive): “As an added validation of this model, we used GFP reconstituted across the synapse (GRASP [34, 35]) to visualize connections between these two cell types (Figure S2).”
Activity of Defined Mushroom Body Output Neurons Underlies Learned Olfactory Behavior in Drosophila (2015, methodology): “Although GRASP is most reliably a proximity marker, it is notable that the GRASP visible in the SMP appears to preferentially label terminals of M4/6 neurons rather than the non-synaptic neurites, suggesting that the points of contact may be genuinely synaptic.”
Genetic manipulation of genes and cells in the nervous system of the fruit fly (2011, background): “A system called GRASP (GFP reconstitution across synaptic partners) that detects cell-cell contact such as that which occurs at synapses has been developed in C. elegans and imported to Drosophila (Feinberg et al., 2008; Gordon and Scott, 2009).”
Motor control in a Drosophila taste circuit (2009, methodology): “To further examine this possibility, we adapted the split-GFP approach recently developed in C. elegans (Feinberg et al., 2008).”
Cell-type Specific Labeling of Synapses in vivo through Synaptic Tagging with Recombination (STaR) (2014, methodology): “elegans (Feinberg et al., 2008) and limitations we encountered in using it to study synapse formation in the fly visual system.”