Simultaneous visualization of multiple protein interactions in living cells using multicolor fluorescence complementation analysis

By Chang‐Deng Hu, T. Kerppola. Nature Biotechnology, 2003

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Fluorescent proteins and their applications in imaging living cells and tissues. (2010, background): “A very important step forward was the multicolor BiFC assay (179).”
Visualization of protein interactions in living plant cells using bimolecular fluorescence complementation. (2004, background): “Furthermore, by introducing a large numberof differentGFPvariants the techniquewasextended to multicolor BiFC, which allows the direct visualization of multiple protein interactions within the same cell (Grinberg et al., 2004; Hu and Kerppola, 2003).”
Bimolecular fluorescence complementation (BiFC) analysis as a probe of protein interactions in living cells. (2008, methodology): “The validity of results from BiFC analysis must be confirmed by examining fluorescence complementation by fusion proteins in which the interaction interface has been mutated (37,45,46).”
Protein tagging and detection with engineered self-assembling fragments of green fluorescent protein (2005, background): “, but the GFP fragments reported thus far are large and fold poorl”