A Novel Mammalian Iron-regulated Protein Involved in Intracellular Iron Metabolism*

By S. Abboud, D. Haile. Journal of Biological Chemistry, 2000

1,291 citations recorded by the source corpus.

We have isolated and characterized a novel iron-regulated gene that is homologous to the divalent metal transporter 1 family of metal transporters. This gene, termed metal transporter protein (mtp1), is expressed in tissues involved in body iron homeostasis including the developing and mature reticuloendothelial system, the duodenum, and the pregnant uterus. MTP1 is also expressed in muscle and central nervous system cells in the embryo. At the subcellular level, MTP1 is localized to the basolateral membrane of the duodenal epithelial cell and a cytoplasmic compartment of reticuloendothelial system cells. Overexpression of MTP1 in tissue culture cells results in intracellular iron depletion. In the adult mouse, MTP1 expression in the liver and duodenum are reciprocally regulated. Iron deficiency induces MTP1 expression in the duodenum but down-regulates expression in the liver. These data indicate that MTP1 is an iron-regulated membrane-spanning protein that is involved in intracellular iron metabolism.

Subfields: Genetics, Hematology, Nutrition and Dietetics

View Paper: https://www.semanticscholar.org/paper/81db473b2d7418c622605c914d6b729696083484

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In Genetics

1. T. Ganz
2. V. Haase
3. Kostas Pantopoulos
4. C. Wood
5. C. Brauner
6. A. Farrell
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In Hematology

1. T. Ganz
2. V. Haase
3. Kostas Pantopoulos
4. G. Papanikolaou
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6. C. Brauner
7. A. Farrell
8. D. Richardson

In Nutrition and Dietetics

1. T. Ganz
2. C. Wood
3. C. Brauner
4. A. Farrell
5. C. Bridges
6. G. Papanikolaou
7. Kostas Pantopoulos
8. D. Templeton

Citations in the corpus

• Regulation of iron homeostasis by the hypoxia-inducible transcription factors (HIFs). (2007, background): “While ferroportin expression in the duodenum could result from the decrease in systemic hepcidin expression or from the systemic iron deficiency itself (25), Figure 3 Binding of HIF-1 to the promoter of hepcidin and downregulation of hepcidin in Albumin-Cre/VHLflox/flox.”
• Iron in Infection and Immunity (2013, background): “N IH -PA Author M anuscript N IH -PA Author M anuscript (Abboud and Haile, 2000; Donovan et al., 2000; McKie et al., 2000).”
• Homeostasis and toxicology of essential metals (2012, background): “However, Fe leaves the cell via the ferrous iron exporter slc40 a1, more commonly known as ferroportin or IREG1 (Abboud and Haile, 2000; Donovan et al., 2000; McKie et al., 2000), which is coupled to the membrane bound multi-copper ferroxidase hephaestin (Vulpe et al., 1999) that facilitates the oxi”
• Molecular and ionic mimicry and the transport of toxic metals. (2005, background): “, 1994), the metal transport protein, MTP1 (Abboud and Haile, 2000) and amino acid or peptide transporters that are localized in the sinusoidal plasma membrane.”
• The iron exporter ferroportin/Slc40a1 is essential for iron homeostasis. (2005, background): “The ferroportin mRNA contains an iron regulatory element in its 5# untranslated region (Abboud and Haile, 2000; Donovan et al., 2000; Liu et al., 2002; McKie et al., 2000).”
• Systemic iron homeostasis. (2013, background): “The sole known mammalian iron exporter is ferroportin [also called Scl40a1, iron-regulated gene 1 (IREG1), or metal transporter protein 1 (MTP1)] (1, 59, 151).”
• Molecular and Cellular Iron Transport (2002, background): “More subtle structural differences at the site may explain the different rates of the formation and decay of the peroxodiferric intermediate, such as the ones observed between tadpole H and M ferritins (49).”
• Identification of an intestinal heme transporter. (2005, background): “…(Raffin et al., 1974), which then enters the low molecular weight pool of iron in the enterocyte, along with iron absorbed as inorganic (i.e., non-heme) iron, and exits the enterocyte via the basolateral transporter ferroportin (McKie et al., 2000; Donovan et al., 2000; Abboud and Haile, 2000).”
• Chemistry and biology of eukaryotic iron metabolism. (2001, background): “Iron export: ferroportin 1, Ireg1, MTP1 The transporter responsible for the export of iron across membranes has also been recently identified in three different laboratories.”