Dissection of the Autophagosome Maturation Process by a Novel Reporter Protein, Tandem Fluorescent-Tagged LC3

By Shunsuke Kimura, Takeshi Noda, T. Yoshimori. Autophagy, 2007

2,226 citations recorded by the source corpus.

Subfields: Cell Biology, Epidemiology, Physiology

View Paper: https://www.semanticscholar.org/paper/7e02bb11d2aa1f59bd1049ac05b6854da638c09b

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Citations in the corpus

Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition) (2012, positive): “orophore such as mRFP or mCherry, which retain fluorescence even at acidic pH.(264) On the one hand, a putative advantage of”
Inhibition of PIP4Kγ ameliorates the pathological effects of mutant huntingtin protein (2017, background): “This double tagged LC3 is commonly used to distinguish between autolysosomes and autophagosomes or phagophores (Hundeshagen et al., 2011; Kimura et al., 2007).”
Guidelines for the use and interpretation of assays for monitoring autophagy (2012, background): “Therefore, it is worth considering the use of stable episomal plasmids that avoid the problem of unsuitable integration.(181) An important advantage of transient transfection is that this approach is better for examining the immediate effects of the transfected protein on autophagy, although it rest”
Monitoring and Measuring Autophagy (2017, background): “Taking advantage of the differential sensitivity to lysosomal acidity, mRFP-GFP-LC3 tandem fluorescent-tagged LC3 can be used to visualize transition from neutral autophagosomes to acidic autolysosomes [38].”
Autophagy‐based unconventional secretory pathway for extracellular delivery of IL‐1β (2011, result): “This was apparent from the data obtained with the RFP–GFP–LC3 probe follow- ing published methods (Kimura et al, 2007), which showed reduced GFPþRFPþ LC3 profiles (early autophagosome) and equal number of GFP RFPþ LC3 profiles (mature autophagic organelles) in cells knocked down for GRASP55 (Figure…”
The dynamic nature of autophagy in cancer. (2011, background): “This reporter has GFP–RFP fused to the LC3 protein (Kimura et al. 2007).”
Pathogenic Lysosomal Depletion in Parkinson's Disease (2010, neutral): “4F) (Kimura et al., 2007).”
Defective Mitophagy in XPA via PARP1 Hyperactivation and NAD+/SIRT1 Reduction (2014, positive): “Accordingly, quantification of autophagy using an monomeric red fluorescent protein (mRFP)-GFP tandem fluorescent-tagged LC3 plasmid (Kimura et al., 2007) revealed increased numbers of both autophagosomes and autolysosomes compared to controls (Figures S3C–S3E).”