Monitoring and Measuring Autophagy
By Saori R. Yoshii, N. Mizushima. International Journal of Molecular Sciences, 2017
992 citations recorded by the source corpus.
Autophagy is a cytoplasmic degradation system, which is important for starvation adaptation and cellular quality control. Recent advances in understanding autophagy highlight its importance under physiological and pathological conditions. However, methods for monitoring autophagic activity are complicated and the results are sometimes misinterpreted. Here, we review the methods used to identify autophagic structures, and to measure autophagic flux in cultured cells and animals. We will also describe the existing autophagy reporter mice that are useful for autophagy studies and drug testing. Lastly, we will consider the attempts to monitor autophagy in samples derived from humans.
Subfields: Epidemiology, Pharmacology, Public Health, Environmental and Occupational Health
View Paper: https://www.semanticscholar.org/paper/d3f5c5c3fedbc86fb4c62bb1de585e4256835f66
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Citations in the corpus
- Autophagy-Dependent Ferroptosis: Machinery and Regulation (2020, background): “Thus, autophagic flux capacity refers to the balance between the formation and degradation of autophagosomes or MAP1LC3-II during a certain time period (Yoshii and Mizushima, 2017).”
- Measuring biological aging in humans: A quest (2019, background): “…by quantifying accumulation of autophagosome cargo upon inhibition of lysosomal proteolysis, are more reliable and the only suitable assay to discriminate whether increase abundance of autophagosomes is due to increase biogenesis or to reduced clearance (Klionsky, 2014; Yoshii & Mizushima, 2017).”