Guidelines for the use and interpretation of assays for monitoring autophagy

By D. Klionsky, Fábio Camargo Abdalla, H. Abeliovich, Robert T. Abraham, Abraham Acevedo-Arozena, K. Adeli, Lotta Agholme, M. Agnello, P. Agostinis, J. Aguirre-Ghiso and 985 more. 2012

4,856 citations recorded by the source corpus.

Subfields: Epidemiology, Parasitology, Plant Science

View Paper: https://www.semanticscholar.org/paper/e32ad6586854e69af86fd3250f7c3091957fdc3d

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Epidemiology

1. Guido Kroemer
2. L. Galluzzi
3. D. Tang
4. R. Kang
5. I. Slaninová
6. Alec C. Kimmelman
7. L. Zitvogel
8. Oliver Kepp

In Parasitology

1. L. Galluzzi
2. Marina Colombo
3. Guido Kroemer
4. R. Kang
5. Alec C. Kimmelman
6. D. Tang

In Plant Science

1. L. Galluzzi
2. Alec C. Kimmelman
3. H. Plun-Favreau
4. Paul Denny
5. Guido Kroemer
6. David P. Hill
7. R. Kang
8. Marc Feuermann

Citations in the corpus

• Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition) (2012, neutral): “determine which criteria are essential for demonstrating autophagy, either for the purposes of their own research, or in the capacity of a manuscript or grant review.(1) Acceptable”
• Consensus guidelines for the detection of immunogenic cell death (2014, background): “The latter not only mediate direct antineoplastic effects, mostly by secreting interferon γ (IFNγ) and via the granzyme-perforin pathway, but also underlie the establishment of protective immunological memory (Figure 1).(231)”
• Molecular definitions of autophagy and related processes (2017, neutral): “Both LC3 lipidation and GFP-LC3 + cytoplasmic dots, indeed, are relatively reliable indicators of the pool size of the autophagosomal compartment, which is known to expand in the course of productive macroautophagic responses (increased on-rate) (Klionsky et al , 2016).”
• WIPI2 Links LC3 Conjugation with PI3P, Autophagosome Formation, and Pathogen Clearance by Recruiting Atg12–5-16L1 (2014, background): “Levels of SQSTM-1/p62, an autophagy cargo receptor, are used to monitor autophagic flux (Klionsky et al., 2012).”
• The GCN2‐ATF4 pathway is critical for tumour cell survival and proliferation in response to nutrient deprivation (2010, positive): “…VOL 29 | NO 12 | 2010 &2010 European Molecular Biology Organization2086 autophagy marker p62/SQSTM1, a long-lived protein that is rapidly degraded during autophagy progression (Klionsky et al, 2008). p62 was degraded in shNT cells on Gln deprivation but stabilized in shGCN2 cells (Figure 6F).”
• Phosphatidylinositol-5-phosphate 4-kinases regulate cellular lipid metabolism by facilitating autophagy (2018, background): “This increase could result from an enhanced induction of autophagy, a block in the turnover of LC3-bound autophagosomes, or both (Klionsky et al., 2012).”