Site-specific labeling of cell surface proteins with biophysical probes using biotin ligase

By Irwin Chen, M. Howarth, Weiying Lin, A. Ting. Nature Methods, 2005

611 citations recorded by the source corpus.

Subfields: Molecular Biology, Organic Chemistry, Radiology, Nuclear Medicine and Imaging

View Paper: https://www.semanticscholar.org/paper/7d343fe5d368cd0091812170dccc7334cc840be5

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Citations in the corpus

Next-Generation Optical Technologies for Illuminating Genetically Targeted Brain Circuits (2006, background): “A range of new methods have been developed for targeting probes (including fluorophores, photoaffinity labels, and quantum dots) to specific proteins in living neurons (Chen et al., 2005).”
Super resolution fluorescence microscopy (2009, background): “can be covalently linked to dye molecules through enzymatic reactions [such as those catalyzed by biotin ligase (76), liopoic acid ligase (77), and sortase (78)].”
Click Chemistry in Complex Mixtures: Bioorthogonal Bioconjugation (2014, background): “, 2009), and use in peptide tags that direct enzymatic ligation of aldehyde or ketone bearing small molecules (Chen et al., 2005; Rashidian et al., 2012).”
Site-specific biotinylation of purified proteins using BirA (2015, background): “4 including desthiobiotin for reversible streptavidin binding (19), or analogs containing functional groups for bio-orthogonal reaction: keto (20), azido and alkyne groups (21).”
Whole cell 3D STORM reveals interactions between cellular structures with nanometer-scale resolution (2008, neutral): “For example, the linked dye can be coupled to an enzymatic substrate, which can then be ligated to a specific peptide sequence fused to a protein of interest, allowing genetic targeting of these bright photoswitchable probe”
Systematic exploration of ubiquitin sequence, E1 activation efficiency, and experimental fitness in yeast (2014, methodology): “The yeast E1 (Uba1) open reading frame was cloned with a biotin ligase acceptor peptide 73; 74 encoded at the far C-terminus into a pAC-T7, an expression vector with a T7 promoter and a chloramphenicol resistance marker.”