Whole cell 3D STORM reveals interactions between cellular structures with nanometer-scale resolution
By Bo Huang, S. A. Jones, Boerries Brandenburg, X. Zhuang. Nature Methods, 2008
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The ability to directly visualize nanoscopic cellular structures and their spatial relationship in all three dimensions will greatly enhance our understanding of molecular processes in cells. Here we demonstrated multicolor three-dimensional (3D) stochastic optical reconstruction microscopy (STORM) as a tool to quantitatively probe cellular structures and their interactions. To facilitate STORM imaging, we generated photoswitchable probes in several distinct colors by covalently linking a photoswitchable cyanine reporter and an activator molecule to assist bioconjugation. We performed 3D localization in conjunction with focal plane scanning and correction for refractive index mismatch to obtain whole-cell images with a spatial resolution of 20–30 nm and 60–70 nm in the lateral and axial dimensions, respectively. Using this approach, we imaged the entire mitochondrial network in fixed monkey kidney BS-C-1 cells, and studied the spatial relationship between mitochondria and microtubules. The 3D STORM images resolved mitochondrial morphologies as well as mitochondria-microtubule contacts that were obscured in conventional fluorescence images.
Subfields: Biophysics, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/5f37da35235f2f1a521779dcedd1704157b41a9e
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Citations in the corpus
- Nuclear pores as versatile reference standards for quantitative superresolution microscopy (2019, unknown): “Cellular reference structures have included histones 1 , mitochondria 11 , and microtubules 12 .”
- Super resolution fluorescence microscopy (2009, background): “Combining with zscanning allowed whole mammalian cells (several micrometers thick) to be imaged (53).”
- Fluorophore localization algorithms for super-resolution microscopy (2014, methodology): “Consider, for instance, a linear cytoskeletal filament, a commonly imaged feature in localization microscopy 24 ( Fig.”
- Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics (2015, result): “were both comparable with similar data from a fixed cell imaged with 3D localization microscopy (45) at a reported xyz resolution of ~20 by 20 by 60 nm (fig.”