CRISPR-P: a web tool for synthetic single-guide RNA design of CRISPR-system in plants.

By Yang Lei, Li Lu, Haiyang Liu, Sen Li, Feng Xing, Lingling Chen. Molecular Plant, 2014

688 citations recorded by the source corpus.

Subfields: Business and International Management, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/7a53f047fae337d15ce328acd53c7fbbd71c4df0

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Citations in the corpus

Evaluation of off-target and on-target scoring algorithms and integration into the guide RNA selection tool CRISPOR (2016, background): “Although published tools and scoring systems allow ranking sequences by specificity [10–22] and efficiency [2, 8, 23–25], they are usually limited to a handful of genomes and only few evidence-based recommendations exist to optimize offtarget search parameters and on-target efficiency.”
Cas-Designer: a web-based tool for choice of CRISPR-Cas9 target sites (2015, background): “…sites for Cas9 nucleases (Aach et al., 2014; Cradick et al., 2014; Doench et al., 2014; Bae et al., 2014a; Heigwer et al., 2014; Hsu et al., 2013; Lei et al., 2014; Montague et al., 2014; O'Brien and Bailey, 2014; Naito et al., 2014; Sander et al., 2010; Upadhyay and Sharma, 2014; Xie et al.,…”
Genome Editing—Principles and Applications for Functional Genomics Research and Crop Improvement (2017, background): “off-target effects in genome editing (Lei et al., 2014; Montague et al., 2014; Lee et al., 2016; Rastogi et al., 2016).”
Development of broad virus resistance in non-transgenic cucumber using CRISPR/Cas9 technology. (2016, methodology): “Cas9/sgRNA1 off-targets were evaluated by the CRISPR-P program (Lei et al., 2014) using the sgRNA1 sequence against the cucumber genome.”
A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and Dicot Plants. (2015, background): “Furthermore, this risk of offtargeting can be minimized by selection of highly specific target sequences by genome searching (Lei et al., 2014; Xie et al., 2014) (see Methods), and unwanted off-targeted mutations in plants can be eliminated, if necessary, by crossing (and backcrossing) the mutant…”
Engineering Quantitative Trait Variation for Crop Improvement by Genome Editing. (2017, methodology): “For theCRISPR/Cas9 construct carrying eight gRNAs targeting the promoters ofSlCLV3, S andSP, eight potential 20 bp sites were selected for gRNA design within a region of 2 Kbp (SlCLV3 and SP) and 4 Kbp (S) upstream of the start codon (ATG) of each gene using the CRISPR-P tool (Lei et al., 2014).”