Tracking adipogenesis during white adipose tissue development, expansion and regeneration

By Qiong A. Wang, Caroline Tao, Rana K. Gupta, P. Scherer. Nature Medicine, 2013

1,195 citations recorded by the source corpus.

White adipose tissue displays high plasticity. We developed a system for the inducible, permanent labeling of mature adipocytes that we called the AdipoChaser mouse. We monitored adipogenesis during development, high-fat diet (HFD) feeding and cold exposure. During cold-induced 'browning' of subcutaneous fat, most 'beige' adipocytes stem from de novo–differentiated adipocytes. During HFD feeding, epididymal fat initiates adipogenesis after 4 weeks, whereas subcutaneous fat undergoes hypertrophy for a period of up to 12 weeks. Gonadal fat develops postnatally, whereas subcutaneous fat develops between embryonic days 14 and 18. Our results highlight the extensive differences in adipogenic potential in various fat depots.

Subfields: Biochemistry, Epidemiology, Physiology

View Paper: https://www.semanticscholar.org/paper/7a2e77544afe650115f10cd92fb0794165ef1823

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Biochemistry

1. B. Spiegelman
2. S. Kajimura
3. E. Rosen
4. L. Sidossis
5. B. Palmer
6. D. Clegg
7. P. Seale
8. P. Scherer

In Epidemiology

1. B. Spiegelman
2. S. Kajimura
3. R. Locksley
4. E. Rosen
5. L. Sidossis
6. A. Chawla
7. P. Seale
8. J. Odegaard

In Physiology

1. B. Spiegelman
2. S. Kajimura
3. L. Sidossis
4. P. Seale
5. E. Rosen
6. P. Scherer
7. A. Chawla
8. R. Locksley

Citations in the corpus

• Adipose tissue plasticity in health and disease (2022, background): “…(i.e., de novo beige adipogenesis), (2) phenotypic conversion of mature white adipocytes into beige adipocytes through the activation (or reactivation) of the thermo-genic program, and (3) the proliferation of mature beige adipocytes (Park et al., 2021; Shao et al., 2019; Wang et al., 2013).”
• Adipose Tissue Dysfunction as Determinant of Obesity-Associated Metabolic Complications (2019, background): “In vivo data confirm these observations in AdipoChaser mice, a model to track adipogenic footpath in vivo [44].”
• Brown and beige fat in humans: thermogenic adipocytes that control energy and glucose homeostasis. (2015, positive): “On the other hand, Scherer’s group performed pulse-chase fate-mapping experiments of mature adipocytes and found that the majority of newly developed beige adipocytes stem from de novo differentiation of precursors in subcutaneous WAT (44).”
• A switch from white to brown fat increases energy expenditure in cancer-associated cachexia. (2014, background): “Recent studies have shown that newly induced beige/brite adipocytes, induced in WAT by cold exposure or adrenergic b3-agonist treatment, arise by recruitment and de novo differentiation of progenitor cells (Wang et al., 2013).”
• Brown and beige fat: Physiological roles beyond heat-generation (2015, result): “However, in sharp contrast to these results, the Scherer lab, using a Doxycyclineinducible (Adipochaser) system to label mature adipocytes, found that a large proportion of cold- or b-agonist-induced beige cells do not come from pre-existing adipocytes (Wang et al., 2013).”
• Adipose Tissue Remodeling: Its Role in Energy Metabolism and Metabolic Disorders (2016, background): “In obese animal models, it has been proposed that de novo adipogenesis begins when the adipocytes reach an upper volume limit (51, 71).”
• Metabolism Disrupting Chemicals and Metabolic Disorders (2016, positive): “Adult mice that are challenged with a high fat diet accumuate fat by hypertrophy in most adipose depots, with the exception f gonadal (visceral) fat which possesses higher capacity to expand y hyperplasia [107].”
• Adipose Tissue Distribution, Inflammation and Its Metabolic Consequences, Including Diabetes and Cardiovascular Disease (2020, background): “Importantly, subcutaneous WAT does not undergo hyperplasia, and hypertrophy lags behind the visceral compartment, with evidence of subcutaneous WAT hypertrophy after 2 months of high fat feeding (170).”
• What We Talk About When We Talk About Fat (2014, positive): “Other recent data also support a role for both hyperplasia and hypertrophy upon high-fat feeding (Wang et al., 2013).”
• The sexual dimorphism of obesity (2015, positive): “Studies in rodents suggest the visceral fat expands predominantly by adipocyte hypertrophy, while the subcutaneous depot expands by adipocyte hyperplasia following exposure to a high-fat diet (Wang et al., 2013).”
• Adipocyte Inflammation is Essential for Healthy Adipose Tissue Expansion and Remodeling (2014, methodology): “Removal of doxycycline enables the detection of newly differentiated adipocytes that are negative for the blue X-Gal-LacZ staining (Wang et al., 2013).”
• Activated Type 2 Innate Lymphoid Cells regulate Beige Fat Biogenesis (2014, positive): “Although the stages of scWAT development have been studied previously (Wang et al., 2013), the physiologic signals that regulate the proliferation and/or fate of scWAT APs were unknown.”