Nascent RNA Sequencing Reveals Widespread Pausing and Divergent Initiation at Human Promoters

By Leighton J. Core, J. Waterfall, J. Lis. Science, 2008

2,019 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/7725b0ccfc61b554cb55cf01b7bb33e7e4bf598c

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In Molecular Biology

Citations in the corpus

Integrative annotation of human large intergenic noncoding RNAs reveals global properties and specific subclasses. (2011, background): “Unstable, likely noncoding, transcripts can also be derived from divergent (bidirectional) transcription in both yeast and mammals (Core et al. 2008; Preker et al. 2008; Seila et al. 2008).”
R-loop formation is a distinctive characteristic of unmethylated human CpG island promoters (2012, positive): “It is possible that this asymmetry, and subsequent R loop formation, may serve to correct the lack of directionality in the initial steps of transcription (Core et al., 2008; Seila et al., 2008).”
c-Myc regulates transcriptional pause release (2010, background): “Promoter-proximal pausing also provides a mechanism to control transcription from bidirectional promoters (Core et al., 2008; Seila et al., 2008), perhaps facilitating the formation of nucleosome-depleted regions and thus providing improved access to regulators (Gilchrist et al., 2008).”
Transcriptional Regulation and its Misregulation in Disease (2013, positive): “Furthermore, new technologies allow investigators to monitor RNA polymerase II molecules that are actively engaged in transcription (Core et al., 2008).”
The many pathways of RNA degradation. (2009, background): “High levels of short, cryptic antisense transcripts that are targets for the exosome are generated from promoter regions in human cells (Core et al., 2008; He et al., 2008; Preker et al., 2008; Seila et al., 2008) and yeast (Davis and Ares, 2006) (Neil et al.”
RNA sequencing: advances, challenges and opportunities (2010, background): “The first involves the ligation of adaptors in a predetermined orientation to the ends of RNAs or to first-strand cDNA molecule”
CpG islands – ‘A rough guide’ (2009, background): “Furthermore, global run on expression analysis indicated that bidirectional transcription initiation frequently occurs at gene promoters [48].”