RNA sequencing: advances, challenges and opportunities

By Fatih Ozsolak, P. Milos. Nature reviews genetics, 2010

2,132 citations recorded by the source corpus.

In the few years since its initial application, massively parallel cDNA sequencing, or RNA-seq, has allowed many advances in the characterization and quantification of transcriptomes. Recently, several developments in RNA-seq methods have provided an even more complete characterization of RNA transcripts. These developments include improvements in transcription start site mapping, strand-specific measurements, gene fusion detection, small RNA characterization and detection of alternative splicing events. Ongoing developments promise further advances in the application of RNA-seq, particularly direct RNA sequencing and approaches that allow RNA quantification from very small amounts of cellular materials.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/cf2841f590819568ab156862c2506ab633493747

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Citations in the corpus

Database resources of the National Center for Biotechnology Information (2017, background): “Expression profiles are both useful complements to already characterized gene functions and also potential means of initially characterizing the function of novel genes (4,5).”
In silico prediction of splice-altering single nucleotide variants in the human genome (2014, background): “A possible solution is to take advantage of the ability of whole transcriptome shotgun sequencing (RNA-seq) to detect alternative splicing (51,52) to recruit variants that do not cause any alternative splicing.”
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