The many pathways of RNA degradation.

By J. Houseley, David Tollervey. Cell, 2009

1,197 citations recorded by the source corpus.

From the earliest comparisons of RNA production with steady-state levels, it has been clear that cells transcribe more RNA than they accumulate, implying the existence of active RNA degradation systems. In general, RNA is degraded at the end of its useful life, which is long for a ribosomal RNA but very short for excised introns or spacer fragments, and is closely regulated for most mRNA species. RNA molecules with defects in processing, folding, or assembly with proteins are identified and rapidly degraded by the surveillance machinery. Because RNA degradation is ubiquitous in all cells, it is clear that it must be carefully controlled to accurately recognize target RNAs. How this is achieved is perhaps the most pressing question in the field.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/500ffd9923783617039262b0fa00d71e366809ef

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Molecular Biology

Citations in the corpus

Ribosome Biogenesis in the Yeast Saccharomyces cerevisiae (2013, background): “Houseley and Tollervey have hypothesized a kinetic proofreading mechanism, based on Hopfield’s ideas (Hopfield 1974; Houseley and Tollervey 2009) and similar to that proposed for turnover when spliceosome assembly or pre-mRNA splicing fails to occur properly (Burgess and Guthrie 1993; Staley and…”
Long COVID or Post-acute Sequelae of COVID-19 (PASC): An Overview of Biological Factors That May Contribute to Persistent Symptoms (2021, background): “While identification of SARS-CoV-2 RNA could technically represent “inert” RNA, the possibility is unlikely because inert RNA in the human body is rapidly degraded (Houseley and Tollervey, 2009; Fabre et al., 2014).”
mRNA-lipid nanoparticle COVID-19 vaccines: Structure and stability (2021, positive): “This process requires water and can be catalyzed by nucleases, but also by the mRNA molecule itself and other exogenous factors like Br ø nsted acids and bases (Houseley and Tollervey, 2009; Pogocki and Sch ¨ oneich, 2000).”
XRN 5’→3’ exoribonucleases: Structure, mechanisms and functions (2013, background): “However, studies using other eukaryotic organisms have added to our understanding of the molecular and biological roles of XRNs in multicellular organisms [1,3–8].”
Hfq and its constellation of RNA (2011, background): “Perhaps in vivo the exchange of RNA on Hfq is assisted by an energy-dependent proof-reading step that improves the accuracy of sRNA-mediated processes, analogous to the mechanisms that ensure translational fidelit”
mRNA-based therapeutics — developing a new class of drugs (2014, neutral): “Step 4: termination of translation by degradation of mRNAs is catalysed by exonucleases 225,226 .”