The CRISPR-associated DNA-cleaving enzyme Cpf1 also processes precursor CRISPR RNA
By Ines Fonfara, Hagen Richter, Majda Bratovič, Anaïs Le Rhun, E. Charpentier. Nature, 2016
893 citations recorded by the source corpus.
Subfields: Molecular Biology
View Paper: https://www.semanticscholar.org/paper/7679cffb564754f7e3c4b25f881f85984f13a936
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In Molecular Biology
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Citations in the corpus
- The Biology of CRISPR-Cas: Backward and Forward. (2018, neutral): “The CRISPR array is preceded by an AT-rich leader sequence.”
- Genome Editing—Principles and Applications for Functional Genomics Research and Crop Improvement (2017, background): “Third, in addition to the nuclease activity to cleave double stranded DNA, Cpf1 also functions as an RNase to process the pre-crRNA arrays into mature single crRNAs (Zetsche et al., 2015; Dong et al., 2016; Fonfara et al., 2016).”
- Diverse evolutionary roots and mechanistic variations of the CRISPR-Cas systems (2016, background): “The observation that inactivation of the RuvC domain prevents cleavage of both strands of the target DNA (39, 91) suggests that the novel nuclease is allosterically activated by the RuvC cleavage event.”
- CRISPR-Cas12a has both cis- and trans-cleavage activities on single-stranded DNA (2018, background): “Besides, Cas12a also processes precursor crRNAs to generate mature crRNAs.(7) However, the cleavage activity of Cas12a on single-stranded DNA (ssDNA) targets is less understood.”
- Transcriptome engineering with RNA-targeting Type VI-D CRISPR effectors (2018, positive): “…and found that EsCas13d was solely sufficient to process its matching CRISPR array into constituent guides without additional helper ribonucleases (Figure 1C; Table S1), a property shared by some class 2 CRISPR-Cas systems (East-Seletsky et al., 2016; Fonfara et al., 2016; Smargon et al., 2017).”
- Multiplex gene editing by CRISPR-Cpf1 through autonomous processing of a single crRNA array (2016, background): “novicida (FnCpf1) and two Cpf1 orthologs with activity in mammalian cells, Acidaminococcus Cpf1 (AsCpf1) and Lachnospiraceae Cpf1 (LbCpf1) ( Figure 1b and Supplementary figure 1 ).”