A Virulence Locus of Pseudomonas aeruginosa Encodes a Protein Secretion Apparatus

By J. D. Mougous, M. Cuff, S. Raunser, A. Shen, Min Zhou, Casey A. Gifford, A. Goodman, G. Joachimiak, C. Ordoñez, S. Lory and 3 more. Science, 2006

1,122 citations recorded by the source corpus.

Subfields: Endocrinology, Molecular Biology, Molecular Medicine

View Paper: https://www.semanticscholar.org/paper/74ded404b081da04b84489576fd411ecbb0c6e13

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Citations in the corpus

A Type VI Secretion System of Pseudomonas aeruginosa Targets a Toxin to Bacteria (2010, positive): “Western blotting was performed as described previously (Mougous et al., 2006), with the exception that detection of the Tse proteins required primary antibody incubation in 5% BSA in Tris-buffered saline containing 0.05% v/v Tween 20 (TBST).”
Type VI secretion apparatus and phage tail-associated protein complexes share a common evolutionary origin (2009, background): “The ClpV protein channel as well as other proteins in the T6SS machine are responsible for translocation of Hcp across the bacterial cell envelope to the external milieu (4).”
Biology of Acinetobacter baumannii: Pathogenesis, Antibiotic Resistance Mechanisms, and Prospective Treatment Options (2017, background): “Many bacteria use the T6SS to inject effector proteins, providing a colonization advantage during infection of eukaryotic hosts (Mougous et al., 2006) or to kill competing bacteria (Basler et al.”
Protein Injection Machines in Bacteria (2018, positive): “The inner tube is primarily composed of a stack of Hcp hexamers forming a 1,000 nm tubular structure capped at its membrane-proximal end by a trimer of VgrG, a structure equivalent to the bacteriophage T4 cell-puncturing device (Leiman et al., 2009; Mougous et al., 2006).”
Type VI secretion requires a dynamic contractile phage tail-like structure (2012, positive): “cholerae , VipA and VipB, form tubular structures that can be depolymerized by another T6SS gene product, Clp”
The Hcp proteins fused with diverse extended-toxin domains represent a novel pattern of antibacterial effectors in type VI secretion systems (2017, background): “for both the assembly of a functional T6SS and the export of its effectors.(22,23,26,43) Crystallographic data of Hcp homologs showed that the protein forms hexameric rings, which can stack into a tube.”
Bacterial Secretion Systems – An overview (2016, background): “T6SSs are the most recent bacterial secretion systems to be discovered (99), and therefore, there is still much to learn about their structure and functions.”