The use of squid protein hydrolysate as a protein source in microdiets for gilthead seabream Sparus aurata larvae

By Kolkovski, Tandler. 2000

100 citations recorded by the source corpus.

In the present study, the use of predigested proteins as an ingredient of microdiets offered to gilthead seabream larvae was tested. The protein source (freeze-dried squid powder) was hydrolysed with protease (trypsin and pancreatin). Different levels of raw squid protein and hydrolysate (100% protein, 50% protein/50% hydrolysate, 100% hydrolysate) were added to the microdiets to produce a dietary protein level of 65%. For comparison, cofeeding of Artemia nauplii and microdiet as well as microdiet supplemented with pancreatin were also offered to the larvae. The final average dry weights of 32-day-old larvae were 1.65 ± 0.04 mg, 1.38 ± 0.06 mg and 1.13 ± 0.1 mg, respectively, for larvae cofed 0%, 50% and 100% hydrolysate microdiets and Artemia nauplii. Survival of larvae was not affected by protein source. The survival of larvae cofed Artemia nauplii and microdiet was significantly higher than that of larvae fed exclusively on microdiet (68% and 80%, respectively). These results suggest that the use of hydrolysate (at 50% greater) as a protein source in diets for seabream larvae is not to be recommended.

Subfields: Aquatic Science, Physiology

View Paper: https://www.semanticscholar.org/paper/714d62bbba2008df696d720b8e5db9b1b6d90c82

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Citations in the corpus

Expression and activities of pancreatic enzymes in developing sea bass larvae (Dicentrarchus labrax) in relation to intact and hydrolyzed dietary protein; involvement of cholecystokinin (2004, background): “, 1991), sea bream (Kolkovski and Tandler, 2000), and sea bass (Cahu et al.”
Nutritive value of squid and hydrolyzed protein supplement in shrimp feed (2002, background): “Kolkovski and Tandler (2000) suggested no more than 50% of protein hydrolysate in fish larval feed.”
Ontogeny and physiology of the digestive system of marine fish larvae (2008, background): “In addition, the dietary incorporation of moderate levels of protein hydrolysates, substituting native proteins, improves the larvae development (Carvalho et al., 1997; Kolkovski and Tandler, 2000).”
Enzymatic fish protein hydrolysates in finfish aquaculture: a review (2020, background): “Also, higher amount of free amino acids could alter the absorption of amino acids leading to an increase of amino acid oxidation and reduced retention of dietary protein (Kolkovski & Tandler 2000; Aragao et al. 2004).”
Leaching properties of three different micropaticulate diets and preference of the diets in cod (Gadus morhua L.) larvae (2006, background): “Other experiments performed with either larvae or juveniles show no advantageous effects of dietary protein hydrolysate (Oliva-Teles et al., 1999; Kolkovski and Tandler, 2000; Garcı́aOrtega et al., 2001).”
Nutritional components affecting skeletal development in fish larvae (2003, background): “Higher substitution levels depressed growth and survival, as was also reported in sea bream larvae fed a diet incorporating hydrolysed squid meal (Kolkovski and Tandler, 2000).”
Solubility and peptide profile affect the utilization of dietary protein by common carp (Cyprinus carpio) during early larval stages (2004, background): “, 1999), while others found no effects or negative effects (Kolkovski and Tandler, 2000).”