Application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology

By G. Muyzer, K. Smalla. Antonie van Leeuwenhoek, 2004

2,121 citations recorded by the source corpus.

Subfields: Ecology, Genetics, Physical and Theoretical Chemistry

View Paper: https://www.semanticscholar.org/paper/70bab818d2c884af4801c10cdcde01096b02cfb4

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Citations in the corpus

Atmospheric Movement of Microorganisms in Clouds of Desert Dust and Implications for Human Health (2007, methodology): “Several other widely used types of postamplification analysis include denaturing gradient gel electrophoresis and temperature gradient gel electrophoresis (168).”
Changes in bacterial community structure induced by mycorrhizal colonisation in split-root maize (2003, methodology): “, 2001) and DGGE (polymerase chain reaction-denaturing gradient gel electrophoresis) (Marschner et al., 2001a,b; Muyzer and Smalla, 1998) are increasingly used for microbial community analysis.”
Microbial populations responsible for specific soil suppressiveness to plant pathogens. (2002, background): “Differences in primary sequence composition can be detected under partially denaturing conditions as in denaturingor temperature-gradient gel electrophoresis [DGGE and TGGE; see (141)] or by single-strand conformation polymorphism [SSCP (180)].”
Rhizobium, Bradyrhizobium and Agrobacterium strains isolated from cultivated legumes (2004, methodology): “Molecular methods that complement the traditional microbiological procedures have been effectively adopted for strain identification (Wu and Tranksley 1993; Liu et al. 1997; Muyzer and Smalla 1998; Muyzer 1999).”
A DGGE-cloning method to characterize arbuscular mycorrhizal community structure in soil (2008, background): “In DGGE or TGGE, DNA fragments of the same length but of different sequence can be separated (Muyzer and Smalla, 1998).”
Orchard floor management practices that maintain vegetative or biomass groundcover stimulate soil microbial activity and alter soil microbial community composition (2005, methodology): “Denaturing Gradient Gel Electrophoresis (DGGE) is one method used to study the dynamics and structure of complex bacterial and fungal communities (Muyzer and Smalla 1998; Muyzer 1999; Heuer et al., 2002; Marschner et al., 2001 2002).”
Reduced diversity of the intestinal microbiota during infancy is associated with increased risk of allergic disease at school age. (2011, methodology): “Molecular techniques based on 16S rRNA PCR combined with DGGE are an efficient method to investigate the bacterial biodiversity in fecal samples, and DGGE is a sensitive cultureindependent method widely accepted for bacterial biodiversity studies.(20,23,25) The DGGEmethod only provides a relative as”
Dietary modulation of the human colonic microbiota: updating the concept of prebiotics (2004, background): “These approaches separate amplified DNA fragments of the same size based on the extent of the sequence divergence between different PCR products (Muyzer & Smalla, 1998).”