A Multiplex Pre-Amplification Method that Significantly Improves Microsatellite Amplification and Error Rates for Faecal DNA in Limiting Conditions

By M. Piggott, E. Bellemain, P. Taberlet, A. Taylor. Conservation Genetics, 2004

121 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/703d9c9f57a55d2c5f9f620bc2bde216430370af

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In Oncology

Citations in the corpus

Estimating population size and trends of the Swedish brown bear Ursus arctos population (2011, methodology): “After this, six microsatellite loci (Mu10,Mu23,Mu50,Mu51,Mu59,G10L; Paetkau & Strobeck 1994, Taberlet et al. 1997) and a sex marker (Bellemain&Taberlet 2004) were amplified, following the multiplex preamplification method (Piggott et al. 2004,Bellemain&Taberlet 2004).”
Importance of a pilot study for non-invasive genetic sampling: genotyping errors and population size estimation in red deer (2006, methodology): “*After multiplex PCR (see Piggot et al. 2004 ) a Approximation over several DNA preservation methods”
How to track and assess genotyping errors in population genetics studies (2004, methodology): “Six microsatellite loci (Mu10, Mu23, Mu50, Mu51, Mu59, G10L) were amplified four times following the multiplex preamplification method ( Pigott et al. 2004 ) and the protocol described in Bellemain & Taberlet (2004).”
Mating Strategies in Relation to Sexually Selected Infanticide in a Non‐Social Carnivore: the Brown Bear (2006, methodology): “For feces or hair samples and for each microsatellite, the amplifications were repeated eight times (multiple tubes approach, Taberlet et al. 1996), using the newly defined ‘multiplex pre-amplification’ approach (Piggott et al. 2004).”
An evaluation of field and noninvasive genetic methods for estimating Eurasian otter population size (2009, unknown): “Piggott et al. 2004; Hedmark and Ellegren 2006) is also suggested, as there is some indication that this method may also be helpful for otter faeces (Lampa et al. 2008).”
Estimating Population Size of Elusive Animals with DNA from Hunter‐Collected Feces: Four Methods for Brown Bears (2005, methodology): “The amplification of microsatellites was carried out following the multiplex preamplification method (Piggott et al. 2004).”
Improved noninvasive genotyping method: application to brown bear (Ursus arctos) faeces (2004, methodology): “These problems are now well understood, and different methods have been proposed to limit genotyping errors and their impacts on the subsequent analyses (Schwartz et al . 1999; Mills et al . 2000; Miller et al . 2002; Paetkau 2003; Piggott et al . 2004 ).”
The impact of time and field conditions on brown bear (Ursus arctos) faecal DNA amplification (2007, methodology): “2003) and PCR amplification (Bellemain and Taberlet 2004; Piggott et al. 2004) may increase overall amplifi-”
Genetics and conservation of European brown bears Ursus arctos (2011, background): “Brown bear research has provided field precautions and laboratory methods to deal with these challenges (Bellemain & Taberlet 2004, Bonin et al. 2004, Piggott et al. 2004, Murphy et al. 2007, De Barba & Waits 2009).”