The impact of time and field conditions on brown bear (Ursus arctos) faecal DNA amplification

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Effects of time and rainfall on PCR success using DNA extracted from deer fecal pellets (2010, background): “…associated with fecal DNA include contamination by microorganisms or digested food items, sensitivity to seasonal weather, high PCR-inhibitor to DNA ratios, and relatively high amplification and genotyping errors (Buchan et al. 2005; Maudet et al. 2004; Murphy et al. 2003; Sefc et al. 2003).”
An evaluation of field and noninvasive genetic methods for estimating Eurasian otter population size (2009, background): “wolf, Santini et al. 2007; or brown bear, Murphy et al. 2007 ), success rates are greatly improved by the use of very fresh samples.”
Genetics and conservation of European brown bears Ursus arctos (2011, background): “Brown bear research has provided field precautions and laboratory methods to deal with these challenges (Bellemain & Taberlet 2004, Bonin et al. 2004, Piggott et al. 2004, Murphy et al. 2007, De Barba & Waits 2009).”