In-gel digestion for mass spectrometric characterization of proteins and proteomes

By Andrej Shevchenko, Henrik Tomas, Jan Havlí, Jesper V. Olsen, Matthias Mann. Nature Protocols, 2006

4,972 citations recorded by the source corpus.

Subfields: Molecular Biology, Spectroscopy

View Paper: https://www.semanticscholar.org/paper/6eeee2e9ad27afac19834e2b09d7358e6b363a65

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Citations in the corpus

Synthetic protein scaffolds provide modular control over metabolic flux (2009, methodology): “0 mm × 17 well ( Invitrogen ), and entire gel lanes were cut and used for in-gel trypsin digestion following the protocol as describe”
Decoding signalling networks by mass spectrometry-based proteomics (2010, background): “Before peptides can be analysed by MS, proteins must be digested in ge”
The Protein Composition of Mitotic Chromosomes Determined Using Multiclassifier Combinatorial Proteomics (2010, positive): “Proteins were separated into a high and a low molecular weight fraction by SDS-PAGE, in-gel digested using trypsin (Shevchenko et al., 2006), and fractionated into 30 fractions each using SCX.”
Gram‐positive bacteria produce membrane vesicles: Proteomics‐based characterization of Staphylococcus aureus‐derived membrane vesicles (2009, methodology): “Tryptic digestion was performed as described previously [15].”
Multiplex peptide stable isotope dimethyl labeling for quantitative proteomics (2009, background): “The differentially labeled samples are mixed and digested with a protease (most often trypsin) to obtain samples that can be readily analyzed by the mass spectromete”
A practical recipe for stable isotope labeling by amino acids in cell culture (SILAC) (2006, methodology): “It has also been used to generate stable isotope-labeled peptides on a whole proteome scale for quantification of brain tissu”
The FLS2-associated kinase BIK1 directly phosphorylates the NADPH oxidase RbohD to control plant immunity. (2014, methodology): “The band for His-RbohDNT was excised from SDS-PAGE gel, and in-gel digestion was performed using a well-established protocol with slight modifications (Shevchenko et al., 2006).”
High Sensitivity Crosslink Detection Coupled With Integrative Structure Modeling in the Mass Spec Studio * (2016, methodology): “Approximately 10 (cid:2) g of total protein was separated on SDS-PAGE, and a band corresponding to the crosslinked proteins was excised and subjected to in-gel tryptic digestion using established protocols (42).”
Sodium taurocholate cotransporting polypeptide is a functional receptor for human hepatitis B and D virus (2012, neutral): “Silver stained gel bands were cut, followed by in-gel reduction, alkylation, and trypsin digestion as previously described (Shevchenko et al., 2006) .”