High-fidelity mRNA amplification for gene profiling

By E. Wang, L. Miller, Galen A. Ohnmacht, E. Liu, F. Marincola. Nature Biotechnology, 2000

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A role for Wnt signalling in self-renewal of haematopoietic stem cells (2003, methodology): “RNA was prepared using Trizol ( Invitrogen ) and linearly amplified using a modified Eberwine synthesi”
Functional classification of interferon‐stimulated genes identified using microarrays (2001, methodology): “A total of 1 mg of each RNA was amplified using two rounds of ds-cDNA synthesis followed by T7-driven in vitro transcription [83].”
Identification of genes periodically expressed in the human cell cycle and their expression in tumors. (2002, methodology): “) Total RNA samples from cells collected in the mitotic shake-off experiment, and total reference RNA were first amplified using a modified Eberwine protocol before cDNA synthesis (Wang et al., 2000) then labeled cDNA was prepared from the amplified RNA.”
BMP4 initiates human embryonic stem cell differentiation to trophoblast (2002, methodology): “This was followed by RNA extraction and amplificatio”