Evolutionary optimization of fluorescent proteins for intracellular FRET

By Annalee W. Nguyen, P. Daugherty. Nature Biotechnology, 2005

700 citations recorded by the source corpus.

Subfields: Biophysics, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/686692add7f11d3244a64c01138537c679443dca

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Citations in the corpus

Fluorescent protein FRET: the good, the bad and the ugly. (2007, methodology): “An additional CFP‐ YFP pair, called CyPET and YPET, has been coevolved to maximize FRET in a specific construct [ 43 ]; however, the enhancement of FRET in this pair is due to intrinsic dimerization between the two FPs [44].”
Α-synuclein misfolding and Parkinson's disease. (2012, background): “For example, crowding had only small effects on high-affinity protein–protein complexes [206], while formation of a low-affinity complex between GFP analogs CyPet and YPet [207] was disrupted, and these proteins aggregated instead.”
Progressive activation of CyclinB1-Cdk1 coordinates entry to mitosis. (2010, methodology): “Using cells expressing YPet alone, we did not observe any photoconversion of YFP molecules to CFP-like species under our experimental conditions (Valentin et al, 2005; Thaler et al., 2006).”
A Guide to Fluorescent Protein FRET Pairs (2016, background): “…to ECFP and EFYP upon introduction of weak dimerization mutations [92], and the importance of these mutations was further validated through the engineering of “sticky” EYFP and ECFP with two mutations found in CyPet and YPet, which showed a 16-fold emission ratio change in a protease sensor [92].”
Genetically encoded calcium indicators for multi-color neural activity imaging and combination with optogenetics (2013, background): “…often by mutating positions near the chromophore to improve folding, maturation, brightness, and photostability (Griesbeck et al., 2001; Nagai et al., 2002; Rizzo et al., 2004; Nguyen and Daugherty, 2005; Ai et al., 2006, 2007; Kremers et al., 2006, 2007; Mena et al., 2006; Goedhart et al., 2010).”
Fluorescent proteins and their applications in imaging living cells and tissues. (2010, background): “One of the brightest yellow FPs reported to date is YPet, which also demonstrates enhanced FRET with co-optimized cyan FP CyPet (311).”
A guide to choosing fluorescent proteins (2005, neutral): “Cyan CyPet Daugherty (2) 435 477 18* 59 5.”
Amiloride inhibits macropinocytosis by lowering submembranous pH and preventing Rac1 and Cdc42 signaling (2010, methodology): “We therefore conclude that the exchange factors that activate Rac1/Cdc42 and/or the GTPases themselves are JCB • VOLUME 188 • NUMBER 4 • 2010 560 CFP variant optimized for FRET (Nguyen and Daugherty, 2005), and either the CRIB domain from p21-activated kinase (PBD) published previously (Machacek et ”
Development of an optimized backbone of FRET biosensors for kinases and GTPases (2011, background): “Originally, YPet was reported to enhance FRET without inducing heterodimerization between YPet and CyPet in an intermolecular FRET biosensor (Nguyen and Daugherty, 2005).”