Development of an optimized backbone of FRET biosensors for kinases and GTPases
By N. Komatsu, K. Aoki, M. Yamada, H. Yukinaga, Y. Fujita, Y. Kamioka, M. Matsuda. Molecular Biology of the Cell, 2011
607 citations recorded by the source corpus.
We report an optimized backbone for the rapid development of a highly sensitive intramolecular fluorescence resonance energy transfer (FRET) biosensor, which includes an optimized pair of fluorescent proteins and a long flexible linker ranging from 116 to 244 amino acids in length. With this backbone system, we developed FRET biosensors of PKA, ERK, JNK, EGFR, RSK, S6K, Akt, PKC, Ras, and Rac1.
Subfields: Biophysics, Cell Biology
View Paper: https://www.semanticscholar.org/paper/0c1254dff38c946608b45751d529e579c58798c1
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In Biophysics
Citations in the corpus
- Improving FRET dynamic range with bright green and red fluorescent proteins (2012, unknown): “7,8 ).”
- A Guide to Fluorescent Protein FRET Pairs (2016, methodology): “In another study, dynamic range was improved for intramolecular FRET sensors through using an optimized backbone Eevee [101], which contains a long and flexible linker that renders the sensor “distance-dependent” rather than “orientation-dependent”, as it is difficult to predict and control orientat”