A guide to choosing fluorescent proteins

By Nathan Christopher Shaner, Paul A. Steinbach, Roger Tsien. Nature Methods, 2005

2,983 citations recorded by the source corpus.

Subfields: Biophysics, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/3735836fdce6e13def7f8a34d48043e8d3d6cbd5

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Biophysics

In Molecular Biology

Citations in the corpus

Optical sectioning microscopy (2005, background): “, though newer variants are helping to reduce this proble”
A bright monomeric green fluorescent protein derived from Branchiostoma lanceolatum (2013, background): “1); talin (22) AgeI and BspEI (mouse talin 1 cDNA source: Clare Waterman, NIH; NM_011602.”
Fluorescent protein FRET: the good, the bad and the ugly. (2007, methodology): “Mutations of GFP [26,27] and the discovery of coral-derived proteins [28,29] have led to a broad palette of different colored proteins [ 29 ].”
Two-photon absorption properties of fluorescent proteins (2011, background): “The linear, one-photon absorption (1PA) spectra and extinction coefficients of many fluorescent proteins have been described and reviewed previousl”
Rapid synaptic scaling induced by changes in postsynaptic firing. (2008, background): “EYFP is highly pH sensitive (pKa of 7.0; Shaner et al., 2005), so tagged receptors localized in acidic intracellular compartmentswill have no appreciable fluorescence but when inserted into theplasmamembrane (pH7.”
Activity-driven local ATP synthesis is required for synaptic function (2014, positive): “To calibrate the local photon flux in terms of the number of luciferase enzymes present, luciferase was tagged with the pH-, thermo-, and photo-stable fluorescent protein mCherry (Shaner et al., 2005), thereby allowing ratiometric luminescence to fluorescence (L/F) readouts.”
A Guide to Fluorescent Protein FRET Pairs (2016, background): “5 53 ND 100 monomer [40,50] eqFP650 592 650 65 0.”
Reproducibility: Designing a rigorous microscopy experiment: Validating methods and avoiding bias (2019, positive): “FPs exhibit environmental sensitivities and therefore must be tested in your experimental system (Shaner et al., 2005; Snapp, 2009; Ettinger and Wittmann, 2014; Costantini et al., 2015; Heppert et al., 2016).”