Adult bone marrow is a rich source of human mesenchymal ‘stem’ cells but umbilical cord and mobilized adult blood are not
By S. Wexler, C. Donaldson, P. Denning-Kendall, C. Rice, B. Bradley, J. Hows. British Journal of Haematology, 2003
680 citations recorded by the source corpus.
Summary. In postnatal life, mesenchymal stem cells (MSC) self‐replicate, proliferate and differentiate into mesenchymal tissues, including bone, fat, tendon, muscle and bone marrow (BM) stroma. Possible clinical applications for MSC in stem cell transplantation have been proposed. We have evaluated the frequency, phenotype and differentiation potential of MSC in adult BM, cord blood (CB) and peripheral blood stem cell collections (PBSC). During culture, BM MSC proliferated to confluence in 10–14 d, maintaining a stable non‐haemopoietic phenotype, HLA class‐1+, CD29+, CD44+, CD90+, CD45–, CD34– and CD14 through subsequent passages. Using the colony forming unit fibroblasts assay, the estimated frequency of MSC in the BM nucleated cell population was 1 in 3·4 × 104 cells. Both adipogenic and osteogenic differentiation of BM MSC was demonstrated. In contrast, CB and PBSC mononuclear cells cultured in MSC conditions for two passages produced a population of adherent, non‐confluent fibroblast‐like cells with a haemopoietic phenotype, CD45+, CD14+, CD34–, CD44–, CD90– and CD29–. In paired experiments, cultured BM MSC and mature BM stroma were seeded with CB cells enriched for CD34+. Similar numbers of colony‐forming units of granulocytes–macrophages were produced by MSC‐based and standard stroma cultures over 10 weeks. We conclude that adult BM is a reliable source of functional cultured MSC, but CB and PBSC are not.
Subfields: Genetics, Hematology, Surgery
View Paper: https://www.semanticscholar.org/paper/5e39e4c32269ed2fc8faa0c6cfc91fadf3988ed4
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Citations in the corpus
- Isolation and characterization of human umbilical cord mesenchymal stem cells with hematopoiesis-supportive function and other potentials. (2006, neutral): “isolation of cord blood-derived MSC are controversial.(14,16) In addition, the process of isolation of MSC is at the expense of losing”
- Aging of mesenchymal stem cell in vitro (2006, neutral): “The low frequency of MSC in BM[7,8] necessitates their in vitro expansion prior to clinical use.”
- Mesenchymal stem cells reside in virtually all post-natal organs and tissues (2006, result): “Since no long-term culture could be derived from circulating blood collected under controlled conditions, MSCs seem to be absent from the circulation under normal physiological conditions, in agreement with Wexler et al. (Wexler et al., 2003).”
- A New Human Somatic Stem Cell from Placental Cord Blood with Intrinsic Pluripotent Differentiation Potential (2004, background): “Several groups were unable to generate MSCs ( 42 , 43) or generated MSCs only in a limited number of CB specimens (39, 41).”