Aging of mesenchymal stem cell in vitro
By M. M. Bonab, K. Alimoghaddam, F. Talebian, S. H. Ghaffari, A. Ghavamzadeh, B. Nikbin. BMC Cell Biology, 2006
876 citations recorded by the source corpus.
BackgroundA hot new topic in medical treatment is the use of mesenchymal stem cells (MSC) in therapy. The low frequency of this subpopulation of stem cells in bone marrow (BM) necessitates their in vitro expansion prior to clinical use. We evaluated the effect of long term culture on the senescence of these cells.ResultsThe mean long term culture was 118 days and the mean passage number was 9. The average number of PD decreased from 7.7 to 1.2 in the 10th passage. The mean telomere length decreased from 9.19 Kbp to 8.7 kbp in the 9th passage. Differentiation potential dropped from the 6th passage on. The culture's morphological abnormalities were typical of the Hayflick model of cellular aging.ConclusionWe believe that MSC enter senescence almost undetectably from the moment of in vitro culturing. Simultaneously these cells are losing their stem cell characteristics. Therefore, it is much better to consider them for cell and gene therapy early on.
Subfields: Genetics, Molecular Biology, Physiology
View Paper: https://www.semanticscholar.org/paper/2a526b299fb0dcccb53430d2e9623d41286ab242
How researchers see this paper
The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.
In Molecular Biology
Citations in the corpus
- Replicative Senescence of Mesenchymal Stem Cells: A Continuous and Organized Process (2008, background): “Our results are in line with similar studies from other groups that demonstrate functional implications on differentiation potential [10,11,34,37].”