Real-time PCR detection of Listeria monocytogenes using an integrated microfluidics platform
By Nathaniel C. Cady, S. Stelick, M. Kunnavakkam, C. Batt. 2005
171 citations recorded by the source corpus.
Subfields: Biomedical Engineering
View Paper: https://www.semanticscholar.org/paper/5bada75098f15b70d12b719996a1440ce28bb865
How researchers see this paper
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In Biomedical Engineering
- D. Weibel
- Willow R Diluzio
- Da Xing
- George M. Whitesides
- Tza-Huei Wang
- Olivier Lazcka
- Samuel Yang
- Wenli Ma
Citations in the corpus
- Pathogen detection: a perspective of traditional methods and biosensors. (2007, background): “Given that fluoescence intensity is proportional to the amount of amplified roduct (Cady et al., 2005), it is possible to follow the amplifiation in real time, thus eliminating laborious post-amplification rocessing steps such as gel electrophoresis.”
- Miniaturized PCR chips for nucleic acid amplification and analysis: latest advances and future trends (2007, background): “The realtime detection kinetically observes the fluorescence signals from the interaction between the dye or probe and the increasing amount of double-stranded DNA molecules during PCR (10, 16, 20, 37, 39, 50, 54, (77) (78) (79) (80) ; the end-point detection measures the fluorescence only before an”
- PCR microfluidic devices for DNA amplification. (2006, methodology): “This bonding technique has been successfully applied to enclose PDMS-based PCR microfluidics with PDMS covers so as to form a reaction channel or chamber where all walls are made from the same material (Cady et al., 2005; Fukuba et al., 2002, 2004; Shin et al., 2003; Yu et al., 2003).”
- Microfabrication meets microbiology (2007, methodology): “A fully automated, microfluidic, PCR-based detection system for the detection of bacterial pathogens was recently describe”
- Advances in Microfluidic PCR for Point-of-Care Infectious Disease Diagnostics (2011, background): “Light emitting diode (LED) and photo-diode (Cady et al. , 2005) are inexpensive alternatives although the illumination power, bandwidth, and the detection sensitivity are limited compared to the conventional laser optic system.”